Fig. 2

Macrophages produce FXIII-A in RA synovial tissues. A–E Dual-fluorescent immunohistochemistry (IHC) for synovial tissues collected from patients with RA. Cell nuclei were stained with acridine orange. A Merged anti-FXIII-A (blue) and anti-FXIII-B (red) antibodies and nuclear staining (green). B–E Anti-FXIII-A antibodies (red), nuclear staining (green), and each cell-surface marker antibody (blue) were merged. B Anti-CDH11 antibody, C anti-CD68 antibody, D anti-CD14 antibody, and E anti-CD163 antibody. F Percentage of positive cells for each antibody among FXIII-A-positive cells (n = 4–5, each). Data are presented as mean ± SEM. A–E Black scale bar = 100 μm