Fig. 3

FXIII-B secretion from fibroblast-like synoviocytes. A, B Merged anti-FXIII-B antibody (red), anti-CDH11 antibody (blue), and nuclear staining (green). Black scale bar = 100 μm. A OA (n = 4) and B RA (n = 4). C Percentage of anti-CDH11 antibody-positive cells among anti-FXIII-B antibody-positive cells on the lining and sublining layers. Data are presented as mean ± SEM. D Analysis of FXIII-B using Western blotting. Culture supernatants obtained from primary cultured FLS (n=2, from two patients with RA), MH7A cell line (immortalized rheumatoid fibroblast-like synoviocytes), HEK293T, and monocyte-derived macrophages were collected and concentrated using the acetone method. Western blotting images were cropped to improve the conciseness of the data