Fig. 4

MXRA7 proteins manifested direct interactions with Vimentin. a Membrane proteins from NIH3T3 fibroblasts were subjected to pull-down, and recovered samples were separated on SDS-PAGE followed by staining for vimentin or GST control. b Total proteins prepared from NIH3T3 cells were precipitated with anti-MXRA7 antibodies or anti-vimentin, respectively, and sediments were cross-examined by western blot. c The co-localization of vimentin and MXRA7 proteins in NIH3T3 cells was detected by a confocal laser microscope. d Vimentin antibody can block the proliferative effect on MXRA7^−/− fibroblasts caused by the protein rmMXRA7