Fig. 3
Increase in immature neutrophils in AppNL-G-F mice with acute colitis. A UMAP visualization of scRNA-seq data of neutrophils. B Developmental trajectory displayed on UMAP and colored by slingshot pseudotime. C Gene expression heatmap of the top 10 signature genes per cluster. D Violin plot of the expression levels of Ly6G, Il1b, and Mki67 in the different cell clusters. E Gene Ontology (GO) analysis of DEGs of neutrophils in cluster2. F UMAP of neutrophils from AppNL-G-F mice under different treatments. G The percentage of Mki67+ cells in neutrophils in cluster 2. H, I Flow cytometric analysis of cells in the blood from WT or AppNL-G-F mice. The percentage of neutrophils in CD11b+ cells (H), and the percentage of neutrophils with high or intermediate/low expression of surface Ly6G (I). Data points are individual mice from one representative experiments. P values were determined by chi-square test (G), one-way ANOVA (H), or two-tailed Mann-Whitney U test (I). Data are shown as the mean ± s.e.m