Fig. 4
Reduction of Aβ plaques in the brains of AppNL-G-F mice with acute colitis by neutrophil depletion. A Schematic diagram of the experimental design to induce acute DSS colitis and deplete neutrophils. AppNL-G-F mice given water or 2% DSS were injected with anti-Ly6G antibody plus anti-rat kappa light chain antibody or isotype control antibody. B Flow cytometric analysis of cells in the blood. C The body weight of the mice. D The colon length of the mice. E Immunohistochemical staining of glial cell activation and Aβ plaques. Representative images of Aβ, and GFAP staining. Scale bar = 1 mm. F The percentage of Aβ plaque area in cortex and hippocampus. Data points are individual mice from one representative of two independent experiments (B–E), and in pooled data of two individual experiments (F). P values were determined by two-tailed Mann-Whitney U test (D, F). Data are shown as the mean ± s.e.m