Fig. 1

Construction of the Wnt3a-diCARs and their signal transduction property in mouse ES cells. A A schematic illustration of wild-type and the chimeric antigen receptors for Wnt3a (Wnt3a-diCAR). Wnt3a-diCAR consists of the single-chain Fv region (scFv) of an anti-fluorescein (FL) antibody and the transmembrane/cytoplasmic domains of LRP6 and Frizzled8 (FZD8). B Molecular structures of wild-type and chimeric LRP6 and FZD8 proteins. An immunoglobulin heavy chain secretion signal sequence (Sig) is placed upstream of each chimera for cell surface expression. The EGF repeats 1–4 (E1–E4), LDLR repeats 1–3 (L1–3), cysteine-rich domain (CRD), and transmembrane domain (TM) are indicated. C TCF/β-catenin-dependent transcriptional activities were measured by luciferase reporter assays in mouse ES cells transiently transfected with various combinations of chimeric LRP6 and FZD8. The signal was normalized to the vehicle-treated mock-transfected cells, which was set to 1. The data represent the mean of duplicates and standard deviation from one representative experiment