Fig. 2

BSA-FL treatment activates TCF/β-catenin-dependent transcription and upregulates the expression of Wnt target genes in ES/scLRP6S/scFZD8L. A, B TCF/β-catenin-dependent luciferase activities in ES/mock and ES/scLRP6S/scFZD8L in response to various concentrations of BSA-FL (A) or Wnt3a (B). Firefly luciferase activities were normalized to Renilla luminescence intensities driven by the constitutively active promoter. The values in ES/mock treated with vehicle were set to 1. The data represent the mean of triplicates and standard deviation from one representative experiment. C A heatmap of Pearson’s correlation coefficient and dendrogram among DNA microarray data from ES/mock and ES/scLRP6S/scFZD8L stimulated with 0, 0.1, or 1 μg/ml BSA-FL, or 50 ng/ml Wnt3a from days 2 to 3 of differentiation. D Venn diagram showing the number of upregulated genes by 1 μg/ml BSA-FL (green) or 50 ng/ml Wnt3a (orange) in ES/mock and ES/scLRP6S/scFZD8L. E GO analysis of the 38 genes upregulated by 1 μg/ml BSA-FL and 35 genes upregulated by 50 ng/ml Wnt3a in ES/scLRP6S/scFZD8L are shown. Red characters indicate the overlapped categories. F Expression levels of Wnt target genes on day 3 of differentiation of ES/scLRP6S/scFZD8L. Cells were stimulated with vehicle, 0.1 μg/ml BSA-FL, or 50 ng/ml Wnt3a from day 2 to day 3. Expression levels of vehicle-treated cells were set to 1. The data represent the mean of duplicates and standard deviation from one representative experiment