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Fig. 3 | Inflammation and Regeneration

Fig. 3

From: Canonical Wnt signaling activation by chimeric antigen receptors for efficient cardiac differentiation from mouse embryonic stem cells

Fig. 3

BSA-FL induces efficient myocardial differentiation from ES/scLRP6S/scFZD8L. A An illustration of a myocardial differentiation procedure for mouse ES cells used in this study. B The incidence of spontaneously beating EBs derived from ES/scLRP6S/scFZD8L from day 7 to day 12 of differentiation. Cells were stimulated with 0.1 μg/ml BSA-FL from day 2 to day 3, day 2 to day 4, or day 2 to day 6 as indicated. The data are shown from a representative experiment. C, D Time course of the incidence of spontaneously beating EBs from ES/mock (C) and ES/scLRP6S/scFZD8L (D) stimulated by various concentrations of BSA-FL, 50 ng/ml Wnt3a, or vehicle for 24 h from day 2 after EB formation. The data represent the mean and standard deviation from 3 to 10 independent experiments. *p < 0.05 (vs vehicle; significance was assessed by Turkey’s multiple comparison test). E Representative immunofluorescence images of EBs derived from ES/scLRP6S/scFZD8L treated with vehicle (left) or BSA-FL (middle) are shown. An anti-cardiac α-actinin antibody was used to detect cardiomyocytes (red) and DAPI was used for nucleus staining (blue, scale bars, 500 μm). F Representative trace of the intracellular Ca2+ change in the beating cluster as indicated by fluorescence intensity

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