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Fig. 4 | Inflammation and Regeneration

Fig. 4

From: Canonical Wnt signaling activation by chimeric antigen receptors for efficient cardiac differentiation from mouse embryonic stem cells

Fig. 4

Quantitative gene expression analysis of mesodermal progenitor and differentiation markers in EBs derived from ES/scLRP6S/scFZD8L. A–D mRNA expression levels of mesodermal progenitor markers on day 6 (A), cardiac and non-cardiac mesodermal markers (B, C), and endodermal markers and ectodermal cell markers (D) on day 13. EBs were derived from ES/mock and ES/scLRP6S/scFZD8L stimulated with vehicle, 0.1 μg/ml BSA-FL, or 50 ng/ml Wnt3a from day 2 to day 3 of differentiation. The expression levels were normalized with those of an internal control (Gapdh). The data represent the mean and standard deviation from 3 to 6 independent experiments. *p < 0.05 (vehicle vs Wnt3a). †p < 0.05 (vehicle vs BSA-FL). ‡p < 0.05 (BSA-FL vs Wnt3a). Significance was assessed by Turkey’s multiple comparison test

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Inflammation and Regeneration

ISSN: 1880-8190

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