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Fig. 5 | Inflammation and Regeneration

Fig. 5

From: Canonical Wnt signaling activation by chimeric antigen receptors for efficient cardiac differentiation from mouse embryonic stem cells

Fig. 5

Antagonizing Wnt3a- and BSA-FL-dependent signaling suppressed TCF/β-catenin-dependent transcription and myocardial differentiation from ES/scLRP6S/scFZD8L. A, B TCF/β-catenin-dependent luciferase reporter activities in ES/scLRP6S/scFZD8L stimulated with vehicle, 0.1 μg/ml BSA-FL or 50 ng/ml Wnt3a in presence or absence of 0.5 mg/ml Dkk1 or 5.4 mg/ml monomeric FL. The values of vehicle-treated cells at the start point on day 3 were set to 1. The data represent the mean of triplicates and standard deviation from one representative experiment. *p < 0.05 (vs Dkk1; significance was assessed by Student’s t test). C–E The incidence of spontaneously beating EBs derived from ES/scLRP6S/scFZD8L stimulated with vehicle, Wnt3a, or Wnt3a plus Dkk1 (C), with vehicle, BSA-FL, or BSA-FL plus Dkk1 (D), and with vehicle, BSA-FL, or BSA-FL plus FL (E) from day 6 to day 12 of differentiation. The data represent the mean and standard deviation from 3 to 10 independent experiments. *p < 0.05 (vs vehicle; significance was assessed by Turkey’s multiple comparison test)

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