Fig. 6
From: Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel
Exosomes secreted by mesenchymal stem cells can efficiently enter and transfer siRNA into macrophages and dendritic cells. Exosomes were isolated from the culture supernatant of hMCSs and labeled with PKH26 or transfected with FAM-conjugated siRel. Labeled exosomes were then co-cultured with mouse splenocytes for 24 h. The percentages of PKH26 (A) and FAM (B) positive T cells, dendritic cells, and macrophages were determined by staining cells with APC-conjugated antibodies against CD3, CD11c, and F4/80 and then analyzed by flow cytometry. Results shown are representative of two independent experiments