Fig. 2
From: A lesion-selective albumin-CTLA4Ig as a safe and effective treatment for collagen-induced arthritis
Design, production, and stability of mAlb-CTLA4Ig. A Structure of the extracellular domain of mCTLA4 (pink). The CDR3-like loop is marked in yellow. To block the binding activity, an N-terminal masking domain (dashed shape) should occupy the space over the CDR3-like loop. B Schematic representations of mAlb-CTLA4Ig constructs. eCTLA4, extracellular domain of CTLA4; P, promoter in the expression vector. C Reducing SDS-PAGE (upper panel) and western blot (lower panel, detected by an anti-mouse Fcγ antibody) analyses of mAlb-CTLA4Ig (left lane) and mCTLA4Ig (right lane) purified from ExpiCHO cells transfected with the expression vectors. D The stability of Alb-CTLA4Ig in DMEM containing 10% fetal bovine sera for 7 days, analyzed by western blot using an anti-mouse Fcγ antibody