Fig. 5
Early muscle regeneration differentiates fast from slow-progressing mice (A-D). Representative immunoblot images (full blots images in Additional file 2) and relative densitometric analysis of Pax7 (A and B), MyoG (A and C), and MyoD (A and D) protein expression in GCM muscles of C57SOD1G93A and 129SvSOD1G93A mice compared with NTG littermates (n = 4-6). Protein levels were normalised on the total amount of protein loaded. Data are reported as mean ± SEM. Significance was calculated with 2-way ANOVA with uncorrected Fisher’s LSD post-analysis (*p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001). E Representative confocal images of embryonal muscle fibres (eMyHC; red) on GCM coronal sections of C57SOD1G93A mice 129SvSOD1G93A mice at pre-symptomatic and onset disease stages, immunostained with laminin (green). F The percentage of embryonal muscle fibres was calculated relative to the total number of muscle fibres and in relation to respective NTG mice (n = 4). Data are expressed as the mean (±SEM). Significance was calculated with 2-way ANOVA with uncorrected Fisher’s LSD post-analysis (****p ≤ 0.0001). G and H The immunohistochemical analysis of central nuclei has been performed on coronal sections of GCM in C57SOD1G93A (G) and 129SvSOD1G93A (H) mice and NTG littermates at pre-symptomatic (PS) and onset (OS) disease stages (n = 4). Percentage of embryonal muscle fibres was calculated relative to the total number of muscle fibres and in relation to the respective NTG. Data are expressed as the mean (±SEM). Significance was calculated with 2-way ANOVA with uncorrected Fisher's LSD post-analysis (**p ≤ 0.01)