Fig. 3
H2 induces myofibroblasts and fibroblasts migration and IFE towards α-SMA+ myoepithelial cell transformation in the proximal wound. A, C, E, J Detection of α-SMA/K14, K5/ki67, Col-1/α-SMA and Vimintin/K14 in the leading edge of the proximal wound at day 3 post-wounding. L Col-1/α-SMA co-expression in the dermal fibroblast of proximal wound at day 3 post-wounding. B, D, F, K and M quantification of double/single positive cells in A, C, E, J and M. G, H DSP transcriptome analysis of D3H proximal wound area (D3H leading edge vs D3H ife) showing the upregulated PPI network, key genes and up-GO biological processes in the keratinocytes of leading edge. White and yellow dotted circles indicate ROI of leading edge and ife, respectively. I Observation of keratinocyte tonofilament morphology in 66% H2 and control groups by electron microscope. N GO-BP enrichment and enriched GO-BP clusters of D3H vs D3C DEGs by using metascape online analysis. White dotted line indicates the boundary between the epithelium and dermis. White arrows in A, C, and E indicate co-positive IFE cells, yellow arrows indicate Vimintin+ (J) fibroblasts or Col-1+/α-SMA+ co-positive myofibroblasts (L). Data in B, D, F, K, and M processed Unpaired T test, and were plotted as Mean ± SEM. *P value < 0.05; **P value < 0.01; ***P value < 0.001; no stars for P value > 0.05; Scale bar in A, C, E, J, and L = 100 μm; Scale bar in I = 1 μm. d, dermis; he, hypertrophic epidermal wound edge; ife, interfollicular epithelium. DSP, digital spatial profiler