Fig. 4

H₂ treatment promoted in vivo early epidermal stem cells proliferation at days 1–3 after wounding. A-C Panoramic scanning of wound edge and the representative immunofluorescence images of K14 (green) and ki67 (red) expression in the leading edge (L, 0–1 mm from wound edge), mid-end (M, 1−2 mm from wound edge) and distal end (D, 2-3 mm from wound edge) of the wound; D−F Statistics of K14⁺/ki67⁺ basal cells and hair follicles in the immunofluorescence. G-I. Representative immunofluorescence of p63 (green) and ki67 (red), K15 (green) and ki67 (red), and Lgr6 (green) and ki67 (red) on skin section showing their expression in the leading edge (L), mid-end (M) and distal (D) of the proximal wound in epidermal and hair follicle cells from 1 to 3 days after wound. J−L. Statistics of p63⁺/ki67⁺, K15⁺/ki67⁺, and Lgr6⁺/ki67⁺ basal cells and hair follicles in the immunofluorescence from 1 to 3 days after wounding. White arrow indicates co-positive basal cell; yellow arrow indicates co-positive follicle cell. Data in D–F, J–L processed two-way ANOVA test, and were plotted as Mean ± SEM. * P value < 0.05; **P value < 0.01; ***P value < 0.001; no stars for P value > 0.05; Scale bar = 100 μm