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Fig. 2 | Inflammation and Regeneration

Fig. 2

From: Extracellular vesicles from immortalized mesenchymal stromal cells protect against neonatal hypoxic-ischemic brain injury

Fig. 2

Intranasal ciMSC-EV delivery provides similar protection from HI-induced tissue loss as EVs from primary MSCs. Postnatal day 9 (P9) C57BL/6 mice were exposed to HI followed by i.n. administration of vehicle (0.9% NaCl, Veh), EVs from primary MSCs (prim), or ciMSC-EVs 1, 3, and 5 days after HI. At P16, histological brain injury was assessed on cresyl violet-stained 20-µm cryostat sections (a, scale bar: 1 mm). Coronal tissue sections at the indicated bregma levels are presented in a to reveal tissue loss in different regions according to the legend provided. Quantification of brain atrophy was performed by measurement of intact areas on tissue sections at a distance of 400 µm between + 1 and − 2.6 mm from bregma. Volumes were calculated for total hemispheres (b), striatum (c), cortex (d), and hippocampus (e). Tissue loss is expressed as the percentage of volume reduction compared to intact contralateral volumes. **p < 0.01, ***p < 0.0001, n = 8–10/group

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