Fig. 5

Adipogenic and osteogenic differentiation of Ir-MSCs. (a) Quantitative measurement of the adipogenic differentiation of lot A of BM-MSCs that were exposed to (white bar) or not exposed to (black bar) 0.1 Gy γ-radiation, as assessed by Oil Red O staining. (b) Representative images are shown. Yellow arrows indicate lipid-laden fat cells. Bars 250 μm. (c) Quantitative measurement of the osteogenic differentiation of lot A of BM-MSCs that were exposed to (white bar) or not exposed to (black bar) 0.1 Gy γ-radiation, as assessed by Alizarin Red S staining. (d) Representative images are shown. Bars 250 μm. (e–g) Adipogenic and osteogenic differentiation of Ir-MSCs at the late phase. (e) Schema of culture of BM-MSCs after γ-irradiation. (f, g) Quantitative measurement of the adipogenic (f) and osteogenic (g) differentiation of lot A of BM-MSCs that were exposed to (white bars) or not exposed to (black bars) 0.1 Gy γ-radiation and then cultured for a further 3 weeks (Ir-MSCs at late phase), as assessed by Oil Red O staining and Alizarin Red S staining, respectively. (a, c, f, g) Data are mean values ± SD. n = 5 per group