Fig. 2

Enhanced IL-34 expression in an inflammatory cyst of the submandibular gland compared to normal salivary gland tissues. Representative data of immunohistochemistry staining of IL-34 in normal salivary gland tissues (a) or inflamed submandibular gland (b). IL-34 staining was performed on sections obtained from five different regions of the surgically resected inflammatory cyst (from S1 to S5) in addition to one sample from the adjacent swollen lymph nodes (S6). H & E staining of sections is shown in the left column, followed by two representative data of IL-34 staining in tissues surrounding the inflammatory cyst. Squares within H & E photography indicate the correspondent positions of IHC data. Scale bars, 20 μM. The specific staining of IL-34 in keratinocytes of normal skin is shown in c at different magnifications as a positive control to confirm the specificity of the anti-IL-34 antibody