Fig. 2
From: Isolation of tissue-resident endothelial stem cells and their use in regenerative medicine
Adipose- and skin-derived E-SP cells possess colony formation ability. a E-SP and E-MP cells were cultured on OP9 feeder cells. These cells were stained with anti-CD31 antibody. b Number of colonies formed by cultured ECs. Data show mean ± SEM; **p < 0.01 (n > 3). c FACS analysis of ECs from liver. The gate surrounded by the red line indicates SP cells. d Liver E-SP and E-MP cells were cultured on OP9 feeder cells. e The number of colonies formed by cultured liver ECs. Data show mean ± SEM; **p < 0.01 (n > 3). f The number of colonies formed by cultured E-SP cells. g EC colonies from E-SP cells derived from different tissues. Dashed boxed area is more highly magnified. Scale bars represent 500 μm in a, d, (g, high powered view); 1 mm in (g, low powered view)