Table 3 The summary of McCabe’s methods (2015)
From: Review: corneal endothelial cell derivation methods from ES/iPS cells
Steps | Dual Smad inhibition | → | Cornea media |
Medium | DMEM/F12, 20%KSR, SB431542 (10 mM), NOGGIN (500 ng/ml), bFGF (8 ng/ml) |  | DMEM/F12, 20%KSR, PDGF-BB (10 ng/ml), DKK-2 (10 ng/ml), bFGF (8 ng/ml) |
Coating | Matrigel-coated well | Matrigel-coated well | |
Duration | 3 days | 14 days |