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Table 3 The summary of McCabe’s methods (2015)

From: Review: corneal endothelial cell derivation methods from ES/iPS cells

Steps Dual Smad inhibition Cornea media
Medium DMEM/F12, 20%KSR, SB431542 (10 mM), NOGGIN (500 ng/ml), bFGF (8 ng/ml)   DMEM/F12, 20%KSR, PDGF-BB (10 ng/ml), DKK-2 (10 ng/ml), bFGF (8 ng/ml)
Coating Matrigel-coated well Matrigel-coated well
Duration 3 days 14 days