Table 4 The summary of Zhao’s methods (2016)
From: Review: corneal endothelial cell derivation methods from ES/iPS cells
Steps | Eye field stem cells differentiation | → | Ocular neural crest stem cells differentiation | → | Corneal endothelial cell induction |
Medium | DMEM/F12, N2, B27, SB431542 (5 μM), LDN193189 (50 nM), IWP2 (1 μM), bFGF (20 ng/ml), etc. |  | DMEM/F12, N2, B27, CHIR99021 (3 μM), 2-phospho-L-ascorbic acid (0.3 mM), etc. |  | HE-SFM, 5% FBS, SB431542 (1 μM), H-1125 (2.5 μM), 2-phospho-L-ascorbic acid (0.3 mM), etc. |
Coating | Matrigel-coated well | Matrigel-coated well | FNC coating mix | ||
Duration | 2 days | ~ 80% confluence | 1 week |