Fig. 1

Intravital two-photon microscopy images of calvarial bone in Col2.3-ECFP/TRAP tdTomato mice. a Representative image of the bone cavity at calvarial bone. Cyan, osteoblasts expressing Col2.3-ECFP; red, osteoclasts expressing TRAP tdTomato; blue, bone tissues (second harmonic generation, SHG). Scale bar, 50 μm. b Magnified images of osteoblasts (region delineated by dotted lines in a) and osteoclasts (region outlined in a) captured at an interval of 60 min. Although the osteoblasts underwent very few morphological changes, the osteoclasts underwent dramatical changes in morphology. Scale bar, 10 μm