Fig. 2

ACE2 and CCN1 are co-expressed in young neurons differentiated from hiPSC-NS/PCs. a and b Co-immunostaining images with neuronal marker βIII tubulin and ACE2 to confirm that the cells are neurons. The neural marker βIII tubulin (green) and ACE2 (red) in neurons differentiated from hiPSC-NS/PCs. b is a high-power field. Scale bars represent 20 μm. c and d Co-immunostaining images with neuronal marker βIII tubulin and CCN1 to confirm that the cells are neurons. The neural marker CCN1 (green) and βIII tubulin (red) in neurons differentiated from hiPSC-NS/PCs. d is a high-power field. Scale bars represent 20 μm. e Immunostaining images of ACE2 (green) and CCN1 (magenta) in neurons. ACE2 (green) and CCN1 (magenta) are co-expressed. Scale bars represent 20 μm. Nuclei were counterstained with Hoechst (blue)