Fig. 1

NLRP3 expression is upregulated by obesity. A The mRNA levels of NLRP3 in the testes of obese mice (n = 6). B Western blot analysis and quantitative results of NLRP3 and ASC in the testes of obese mice (n = 6). (C) NLRP3 mRNA levels in cell populations isolated from the testes of obese mice (n = 6). D The mRNA level of IL-1β in primary SCs isolated from HFD mice (n = 6). E Caspase 1 activity in primary SCs isolated from HFD mice (n = 6). F The mRNA levels of NLRP3 in primary SCs (n = 6). G Western blot analysis and quantitative results of NLRP3 and ASC in primary SCs (n = 6). H, I The mRNA levels of IL-1β and caspase 1 activity in primary SCs (n = 6). J NLRP3 mRNA levels in cell populations isolated from the human testis (n = 5). K Western blot analysis and quantitative results of NLRP3 and ASC in human primary SCs (n = 5). Data are presented as the mean ± SD. For A and F, the statistical analysis was carried out by Student’s two-tailed t test; for others, the statistical analysis was carried out by one-way ANOVA. *P < 0.05 vs the matched control