Fig. 5
From: Diet-induced obesity impairs spermatogenesis: the critical role of NLRP3 in Sertoli cells
ROS production regulates NLRP3 activation in the testis during obesity. A, B The ROS production and MDA content in the testes of the indicated groups (n = 6). C, D The ROS production and MDA content in PA-treated TM4 cells (n = 6). E The ROS production in TM4 cells that were treated with various inhibitors (n = 6). F The mRNA levels of gp91phox and p67phox in the testes of the indicated groups (n = 6). G The phosphorylation of p47phox in the testes of the indicated groups (n = 6). H The activity of NADPH oxidase in the testes of the indicated groups (n = 6). I The mRNA levels of gp91phox and p67phox in cultured TM4 cells at 24 h after PA incubation (n = 6). J The activity of NADPH oxidase in PA-treated TM4 cells (n = 6). K, L The expression of IL-1β in PA-treated TM4 cells (n = 6). M Caspase 1 activity in PA-treated TM4 cells (n = 6). N The mRNA level of MMP-8 in PA-treated TM4 cells (n = 6). O Nuclear NF-κB and NLRP3 activation was detected in PA-treated TM4 cells (n = 6). P The protein expression of occludin in PA-treated TM4 cells (n = 6). Data are presented as the mean ± SD. For A–D and F–J, the statistical analysis was carried out by Student’s two-tailed t test; for others, the statistical analysis was carried out by one-way ANOVA. *P < 0.05 vs the matched control