Fig. 2
From: Marine-derived microbes and molecules for drug discovery
Preparation of the marine microbial extract library. Collection of marine animals from the coasts of Nagasaki Prefecture: A sea urchin, B crab, C lobster, D sea cucumber, E sea anemone, F oyster, and G shrimp. H Isolation of marine microbes from various marine animals. Samples from a variety of marine animals are streaked out onto marine agar plates or seawater plates and marine microbes are isolated. I Preparation of marine microbial extracts. The isolated marine microbes are grown in marine broth or seawater broth and the marine microbial extracts are prepared as shown in Fig. 3. J Development of a marine microbial extract library. Marine microbial extracts are dissolved in DMSO at a concentration of 10 or 100 mg/ml and dispensed into 96-well plates