Fig. 3

Iba1-positive microglia infiltrated layers I to VI of the cerebral cortex (dorsal region) located in the anterior region of the brain (1.0 mm anterior to bregma). a Representative immunostaining images of Iba1-positive cells (red) in layers I to III of the anterior cerebrum in the control, sham, mild SAH, and severe SAH groups. Scale bar, 100 μm for the general figure and 50 μm for the enlarged figure. b Representative immunostaining images of Iba1-positive cells (red) in layers IV to VI of the anterior cerebrum in the control, sham, mild SAH, and severe SAH groups. Scale bar, 100 μm for the general figure and 50 μm for the enlarged figure. c Quantification of the number of Iba1-positive cells in layers I to III of the anterior region of the cerebrum (n = 4, each group, control vs. severe SAH; p = 0.002, sham vs. severe SAH; p < 0.001). d Quantification of the number of Iba1-positive cells in layers IV to VI of the anterior region of the cerebrum (n = 4, each group, control vs. severe SAH; p = 0.003, sham vs. severe SAH; p = 0.01). e Quantification of the number of Iba1-positive cells in all layers of the anterior region of the cerebrum (n = 4, each group, control vs. severe SAH; p < 0.001, sham vs. severe SAH; p < 0.001, mild SAH vs. severe SAH; p = 0.025). Statistical analyses were performed with one-way ANOVA and Tukey–Kramer post hoc tests. The values in the bar graphs represent the mean ± SE. *p < 0.05, **p < 0.01, ***p < 0.001