Fig. 1

Extracellular matrix (ECM) directs the acquisition of developmental and regenerative signature in vitro. A The anatomy of the murine distal small intestine to establish intestinal organoids is shown. B A macroscopic image of the fetal murine intestine used to establish dFEnS is shown. The demarcated area indicates the distal small intestine used for in vitro expansion. Scale bar, 1mm. C A macroscopic image of the fetal mouse at E16 is shown. Scale bar, 1 mm. D Representative images of adult murine distal small intestinal organoids cultured in Matrigel with ENR medium (top left panel), in Matrigel with ENRWN medium (top right panel), in collagen with ENRWN medium (bottom left panel), and fetal murine intestinal organoids in Matrigel with ENRWN medium (dFEnS) (bottom right panel). Scale bar, 200μm. E Flow cytometric analysis of cells from the intestinal organoids in MG-ENR (top left panel), MG-ENRWN (top right panel), COL-ENRWN (bottom left panel), and fetal MG-ENRWN (dFEnS) (bottom right panel) is shown. Diagrams show representative plots for Sca1 in the live EpCAM+ve cell population. F The percentages of Sca1^+ve cells in MG-ENR, MG-ENRWN, COL-ENRWN, and fetal MG-ENRWN organoids are indicated. The diagram shows the average ± S.D. (n=3) for adult intestinal organoids grown in MG-ENR, MG-ENRWN, and COL-ENRWN, and the average (n=2) for fetal MG-ENRWN organoids (dFEnS). G qPCR analysis of different cultures (MG-ENR, MG-ENRWN, COL-ENRWN, and fetal MG-ENRWN (dFEnS)) of the small intestine in biological triplicates for indicated genes are shown as a heatmap