Fig. 1

Synovial tissues express FXIII-A and FXIII-B. A, B Expression levels of A F13A1 and B F13B in whole synovial tissue collected from patients with rheumatoid arthritis (RA), osteoarthritis (OA), and healthy control peripheral blood mononuclear cells (n = 11, 7, and 8, respectively), detected with RT-qPCR. C, D Fluorescent IHC for synovial tissues collected from patients with OA and RA. Cell nuclei were stained with acridine orange. C Anti-FXIII-A antibodies (red) and nuclear staining (green) were merged. D Anti-FXIII-B antibodies (red) and nuclear staining (green) were merged. E The proportion of FXIII-A-positive cells on the lining and sublining layers in OA (n = 8) and RA (n = 16) synovial tissues. F The proportion of FXIII-B-positive cells on the lining and sublining layers in OA (n = 8) and RA (n = 8) synovial tissues. A, B, E, F Data are presented as mean ± standard error of mean (SEM), t test. C, D Black scale bar = 100 μm