Fig. 5

FXIII-B promote chemokine secretion in fibroblast-like synoviocytes. A Western blotting. FLS from two patients with RA were transfected with enhanced green fluorescent protein (EGFP) in vitro transcribed (IVT) mRNA or hemagglutinin tagged (HA-tagged) F13B IVT mRNA, then cultured for 24 h. Western blotting images were cropped to improve the conciseness of the data. B, C, D RNA from FLS was collected and analyzed using next-generation sequencing analysis (RNA-seq) (n = 2). B Volcano plot, C Represents the top 10 upregulated or downregulated genes. D Volcano plot for chemokine-related and anti-apoptotic-related genes. E, F RT-qPCR for comparison between FLS transfected with HA-tagged F13B IVT mRNA or those transfected with EGFP IVT mRNA (n = 3). E Expression levels of CXCL8, CXCL1, CCL3, and CXCL2. F Expression levels of TRAF1 and BCL2A1. G RT-qPCR for comparison between MH7A cells transfected with siRNA against F13B and those transfected with siRNA control (n = 3). E–G Data are presented as mean ± SEM, t test, *p<0.05, **p<0.01