Fig. 2.

NSPCs secret factor(s) enhancing neurosphere formation in an autocrine manner under hypoxic condition. A Experimental procedure of (B). The CM was harvested from E14.5 cortical cell culture for 2 days under the normoxic or the hypoxic condition. Neurosphere formation derived from E14.5 enriched NSPCs was performed under the conventional normal condition with CM. B Quantification of the neurospheres formed under the CM (mean ± SEM, n = 4, * p = 0.0355). C Experimental procedure of (D)-(E). The CM was harvested from enriched NSPC culture for 2 days under the normoxic or the hypoxic condition. Enriched NSPCs was cultured under the CM from the normal O₂ atmosphere. D Microphotographs of neurospheres formed under the CM from the normoxic or the hypoxic enriched NSPC culture. Scale bar = 200 μm. E Quantification of the neurospheres formed under the normoxic or the hypoxic CM (mean ± SEM, n = 3, * p = 0.0270).