Fig. 6.

VEGF-A is expressed in the VZ where NSPCs are confined in the developing cortex. A Vegf-A mRNA expression in the E11.5 and 14.5 brain was detected by in situ hybridization. Right panels indicate magnification of boxed area. Scale bar = 50 μm. B Sense probe was used as negative control. Right panels indicate magnification of boxed area. Scale bar = 50 μm. C Immunohistochemistry by anti-Hif-1a antibody in the E14.5 cortex. The center and right panels indicate magnification of boxed area in the left panel. The center panel shows single color image of Hif1-a. Scale bar = 50 μm. LV, lateral ventricle.