Fig. 1
From: Accelerating corneal wound healing using exosome-mediated targeting of NF-κB c-Rel
Expressions of c-Rel and its inflammatory targets are increased in the cornea of mice with corneal injury. Mouse corneal epithelium was either untreated (n = 6) or scraped (n = 6) as described in the “Materials and methods” section. Corneas were isolated 24 h after epithelial scraping. A Total proteins were prepared from the corneas and c-Rel expression was determined by Western blot. β-actin was used as internal control. The blot was cropped to improve the clarity of the image and full-length blot was provided in the supplementary materials. B Total RNA was isolated from the cornea and mRNA expression of all five NF-κB family members was determined by quantitative RT-PCR. C Corneal tissue extracts were prepared and the production of inflammatory cytokines as indicated in the figure was determined by ELISA. Epi on: epithelium not scraped. Epi off: epithelium scraped off. Results shown are representative of two independent experiments. **P<0.01, ***P<0.001