Fig. 5

IL-1α promoted de novo HF regeneration in 3W mice with a 0.5-cm-diameter wound. A Experimental steps. B Representative pictures of HF regeneration. The red arrow points to the regenerated HF. C If the diameter of the wound is less than 0.5 cm, 3W mice could not regenerate HFs. But IL-1α significantly promoted the de novo HFs regeneration (PBS vs IL-1α, 0.0 ± 0.0 vs 4.400 ± 0.7180, n = 10). D Representative pictures of regenerated HFs. The orange arrows point to the boundary between regenerated and original tissue. The green arrows point to the HF have grown out from the regenerated skin. The blue arrows point to the HF were still growing in the regenerated skin. E Representative photos of Lgr5 HFSCs regeneration in 3W Lgr5 + /mTmG mice. ****P < 0.001