Fig. 2

JAK inhibitor influenced migration of osteoclast precursors to the bone surface. A Representative intravital multiphoton imaging of the migration of osteoclast precursors in calvarial bones in JAK inhibitor (JAKi)-treated LPS-induced mice. Green, CX₃CR1-EGFP. Scale bar, 30 µm (upper panels). The movement of CX₃CR1-EGFP⁺ cells was tracked for 10 min. Colored lines show the cell trajectories (lower panels). B Tracking velocities of EGFP⁺ cells (steady state, n = 795 cells; vehicle, n = 570 cells; JAKi, n = 1091 cells from 5 mice per group). C Representative intravital multiphoton imaging of mature osteoclasts in calvarial bones in JAK inhibitor (JAKi)-treated LPS-induced mice. Red, TRAP-tdTomato⁺ mature osteoclast; blue, bones (second harmonics generation). Scale bar, 50 µm. D Area of TRAP-tdTomato⁺ mature osteoclasts relative to steady state (steady state, n = 35 images; vehicle, n = 39 images; JAKi, n = 36 images from 5 mice per group). Error bars indicate the means ± SDs. Statistical significance was determined by Kruskal-Wallis test. ***P<0.001, ****P<0.0001