Fig. 3

JAK inhibitor decreased expression of Ccr1 in osteoclast precursors under inflammatory conditions. A Heatmap of representative receptors on CX₃CR1-EGFP⁺ cells in JAK inhibitor (JAKi)-treated LPS-induced mice. Green, downregulation; red, upregulation (n = 3 mice per group). B Relative mRNA expression of Ccr1 in CX₃CR1-EGFP⁺ cells (n = 6 mice per group). C Representative histograms (upper) and quantitative mean fluorescence intensity (MFI) (lower) of CCR1 in CX₃CR1-EGFP⁺ cells (vehicle, n = 6; JAKi, n = 5 mice). D Representative intravital imaging of osteoclast precursors of CCR1 antagonist-treated LPS-induced mice. Green, CX₃CR1-EGFP⁺ cells. Scale bar, 30 µm (upper panels). Colored lines show the cell trajectories for 10 min (lower panels). E Mean tracking velocities of CX₃CR1-EGFP⁺ cells (vehicle, n = 589 cells; CCR1 antagonist, n = 1281 cells from 6 mice per group). F Representative intravital imaging of mature osteoclasts. Red, TRAP-tdTomato⁺ cells; blue, bones. Scale bar, 50 µm. G Areas of TRAP-tdTomato⁺ mature osteoclasts relative to vehicle controls (vehicle, n = 51 images; CCR1 antagonist, n = 44 images from 6 mice per group). Statistical significance was determined by two-tailed Student’s t-test (B, C) and Mann-Whitney test (E, G). Error bars indicate the means ± SDs. *P < 0.05, **P < 0.01, ****P<0.0001