Fig. 6
Ex vivo muscle derive satellite cells differentiate fast from slow-progressing mice. A Representative confocal images showing the immunostaining for MF20-MyHC (green) and DAPI (blue) on primary satellite cell (SC) cultures of C57SOD1G93A and 129SvSOD1G93A mice in differentiating medium (DM) for 48 h, compared with NTG littermates (n = 3). Scale bar = 100 μm. B The fusion index was calculated as (no. of nuclei present in MyHC+ cells with two or more nuclei/no. myotubes. Data are reported by means ± SEM. Significance was calculated with one-way ANOVA with uncorrected Fisher’s LSD post-analysis (*p ≤ 0.05, **p ≤ 0.01). C–I Real-time qPCR for MyoD (C), MyoG (D), Tmem8c (E), AchRγ (F), AchRε (G), AchRα1 (H) transcripts in the primary SC cultures of C57SOD1G93A and 129SvSOD1G93A mice compared with NTG littermates (n = 3–6). Data are expressed as the mean (±SEM). Significance was calculated with 2-tailed Student’s t-test (p ≤ 0.05; **p ≤ 0.01)