Fig. 6
Daily topical treatment of H2 dressing significantly promoted cutaneous wound healing. A Left: scheme images of Mg-based H2 dressing in cutaneous wound healing treatment; Middle: scanning electron microscope (SEM) image of Mg microparticles; right: H2 release curves of Mg-based dressing. B Timeline of animal experiments and daily H2 dressing treatment (or normal dressing condition). C Representative wound area images captured every 2 days after modeling. D Comparison of wound closure percentage after modeling between H2 dressing and control groups. E, F H&E staining and Masson staining showing the tissue remodeling 11 days and 20 days post-wounding. Data in D was analyzed by two-way ANOVA test. All of the data are plotted as mean ± SEM. *P value < 0.05; **P value < 0.01; ***P value < 0.001; ****P value < 0.0001; no stars for P value > 0.05; scale bar in A = 2 μm, scale bar in E = 100 μm. Red dotted line in E (H&E staining) indicates the boundary between the epithelium and dermis. Black arrowheads in E and F indicate blood capillaries in the dermal layer of the wound. b, basal layer; d, dermis; hf, hair follicles; ife, interfollicular epithelium; s, scab