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Fig. 3 | Inflammation and Regeneration

Fig. 3

From: A human induced pluripotent stem cell model from a patient with hereditary cerebral small vessel disease carrying a heterozygous R302Q mutation in HTRA1

Fig. 3

Pluripotency validation of SM9-1 iPSCs. a Alkaline phosphatase staining of an SM9-1 iPSC colony. Scale bar, 100 μm. b RT-qPCR analysis to examine the expression levels of pluripotency marker genes in SM9-1 T lymphocytes and SM9-1 iPSCs. Values represent the mean ± standard error of the mean (SEM) of technical triplicates. c Immunocytochemistry of human pluripotency markers. Scale bar, 100 μm. d Schematic summary of culture protocol for spontaneous differentiation. e Immunocytochemistry of three germ layers markers using spontaneously differentiated cells in vitro. Scale bar, 100 μm

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Inflammation and Regeneration

ISSN: 1880-8190

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