Fig. 6

Exogenous PELI2 overexpression rescues the effects of ALKBH5 knockdown on keratinocyte migration. a, b qRT‒PCR showing the mRNA expression of PELI2 (a) and ALKBH5 (b) in HaCaT cells treated with ALKBH5 or NC siRNAs and transfected with PELI2 or NC plasmids for 48 h. The experiments were performed in triplicate, and the relative expression of mRNA is shown as the mean ± SD. One-way ANOVA, ns not significant, *P < 0.05, ****P < 0.0001. The full-length blots are presented in Additional file 8: Fig. S1. c The protein expression of ALKBH5 and PELI2 was determined by WB. d Transwell assays showed the effect of exogenous PELI2 overexpression on the migration of ALKBH5-knockdown or control HaCaT cells. Scale bar: 25 μm. e Statistical analysis of the Transwell assay results. All of the experiments were performed in triplicate, and five random fields were included in the analysis. The percent area of migrated cells is shown as the mean ± SD. One-way ANOVA, ***P < 0.001. Scale bar: 25 μm. f The effect of exogenous PELI2 overexpression on the migration of ALKBH5-knockdown or control HaCaT cells was evaluated by wound scratching assay. Scale bar: 100 μm. g Statistical analysis of wound scratching assays. All of the experiments were performed in triplicate, and three random fields at each time point were included in the analysis. Mann–Whitney test (at 24 h), no significant difference was found between groups of ALKBH5-si1 + p-PELI2 vs. ALKBH5-si1 + p-NC and ALKBH5-si2 + p-PELI2 vs. ALKBH5-si2 + p-NC. One-way ANOVA (at 48 h, 72 h), ***P < 0.001