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Fig. 2 | Inflammation and Regeneration

Fig. 2

From: Placenta mesenchymal stem cell-derived extracellular vesicles alleviate liver fibrosis by inactivating hepatic stellate cells through a miR-378c/SKP2 axis

Fig. 2

Fibrosis induction in liver organoids by TGF-β1 treatment. A The workflow of organoid maintenance and TGF-β1 administration. B After the treatment of TGF-β at different concentrations, the secretion of albumin, IL-1β, TNF-α, and Pro-collagen of liver organoids was detected by ELISA. C The mRNA expression of ALB, ACAT2, COL1A1, and COL3A1 was detected by RT-qPCR. D Immunofluorescence staining of collagen I and α-SMA detected by confocal microscopy. E Semi-quantitative analysis of collagen I staining intensity in D. F Semi-quantitative analysis of α-SMA staining intensity in D. TGF-β1, transformation growth factor- beta; ALB, albumin; α-SMA, α-smooth muscle actin. Bar scale, 50 μm. **P < 0.01

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Inflammation and Regeneration

ISSN: 1880-8190

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