Fig. 4

Migration and differentiation profile of the transplanted hiPSC/NS/PCs. a Representative image of a sagittal section stained for STEM121, which detects the human-specific cytoplasmic antigen in the TP and combination groups. STEM121 + grafted cells were elongated from the rostral and caudal sites. Scale bars, 1000 and 200 μm (enlarged images). b Representative images of neural cells differentiated from graft cells. HNA ⁺ grafted cells merged with ELAVL3/4 (neurons), GFAP (astrocytes), and APC (oligodendrocytes). Scale bar: 20 μm. c Quantitative analyses of the proportions of ELAVL3/4 ⁺, GFAP⁺, and APC⁺ cells among the HNA ⁺-grafted cells. n = 7 each. d Representative images of immunostaining for each group. HNA-positive transplanted cells were stained with Ki67 and Nestin. Nuclei were stained with Hoechst. Scale bar: 20 μm. e Quantitative analyses of proportions of Ki-67⁺ cells and Nestin⁺ area per HNA ⁺-grafted cells. n = 7 each. N.S., not significant according to the Mann‒Whitney U test. Values are the means ± SEMs for c and e