Fig. 2
From: Gene editing technology to improve antitumor T-cell functions in adoptive immunotherapy
CRISPR/Cas9-mediated gene editing. A CRISPR/Cas9 technology can knock out specific genes through DNA double-strand breaks followed by nonhomologous end joining (NHEJ). It can also insert donor oligo by the mechanism of homology-directed repair (HDR). B Accurate selection of genomic sequences targeted by guide RNAs can be improved by combinatorial use of sequence-based prediction and reference to epigenetic profiles at the target site