Skip to main content

Advertisement

Pathophysiology and therapeutic potential of cardiac fibrosis

Article metrics

Abstract

Inflammatory and fibrotic responses to myocardial damage are essential for cardiac repair; however, these responses often result in extensive fibrotic remodeling with impaired systolic function. Recent reports have suggested that such acute phase responses provide a favorable environment for endogenous cardiac regeneration, which is mainly driven by the division of pre-existing cardiomyocytes (CMs). Existing CMs in mammals can re-acquire proliferative activity after substantial cardiac damage, and elements other than CMs in the physiological and/or pathological environment, such as hypoxia, angiogenesis, and the polarity of infiltrating macrophages, have been reported to regulate replication. Cardiac fibroblasts comprise the largest cell population in terms of cell number in the myocardium, and they play crucial roles in the proliferation and protection of CMs. The in vivo direct reprogramming of functional CMs has been investigated in cardiac regeneration. Currently, growth factors, transcription factors, microRNAs, and small molecules promoting the regeneration and protection of these CMs have also been actively researched. Here, we summarize and discuss current studies on the relationship between cardiac inflammation and fibrosis, and cardiac regeneration and protection, which would be useful for the development of therapeutic strategies to treat and prevent advanced heart failure.

Background

The number of deaths from cardiovascular diseases is increasing globally, and cardiac dysfunction is closely associated with increased myocardial fibrosis and loss of cardiomyocytes (CMs). Although cardiac fibrosis plays an essential role in the response to pressure overload and/or cardiac injury such as myocardial infarction (MI), its excessive and prolonged reaction can lead to cardiac diastolic and systolic dysfunction. Therefore, the regulation of inflammation and fibrosis at the appropriate timing and duration is crucial for the preservation or recovery of cardiovascular homeostasis. Currently, the inhibition of the renin–angiotensin system (RAS) using angiotensin-converting enzyme (ACE) inhibitors and angiotensin receptor blockers (ARBs) is the most validated clinical strategy for treating patients with advanced heart failure [1].

Cardiac fibroblasts comprise the largest cell population in the myocardium [2], in terms of cell number, and they play a major role in fibrosis by producing the extracellular matrix (ECM) [3]. Cardiac fibroblasts interact with not only CMs but also with non-CMs, including vascular endothelial cells, smooth muscle cells, and immune cells, via direct and indirect cellular communications in an autocrine or paracrine manner [4] (Fig. 1). Recently, cardiac inflammation and fibrosis have been reported to be associated with the cardiac regenerative ability, which is mainly driven by the division of pre-existing CMs [5]; therefore, the modulation of the function of non-MCs for cardiac protection and regeneration has been actively investigated.

Fig. 1
figure1

Interactions among cardiac cells. Most types of cardiac cells, including CMs, cardiac fibroblasts, macrophages, and endothelial cells, regulate cardiac fibrosis and regeneration in a coordinated manner. Some paracrine factors from fibroblasts, including TGF-β and IGF-1, are known to promote the hypertrophic responses of CMs. The regulation of hypoxic environment and macrophage polarization is a key factor for enhancing crucial angiogenic responses involved in cardiac repair and regeneration

Here, we summarize and discuss current studies on the relationship between cardiac inflammation and fibrosis, and cardiac regeneration and protection, which would be useful for the development of therapeutic strategies for treating patients with advanced heart failure.

Main text

Types of cardiac fibrosis

Cardiac fibrosis is classified into two types: reactive fibrosis and reparative (replacement) fibrosis. Reactive fibrosis, which is characterized by the excessive deposition of ECM in the interstitial or perivascular spaces, is triggered by hemodynamic stress, such as pressure overload, and it is not directly associated with CM death [6, 7]. Reactive fibrosis is considered an adaptive response aimed at normalizing the increased wall stress and preserving the cardiac output. However, excessive fibrosis in interstitial spaces may cause mechanical stiffness, resulting in cardiac diastolic dysfunction, and impairment in electric conduction by forming a barrier between CMs, leading to cardiac systolic dysfunction. In addition, excessive fibrosis in perivascular areas decreases the flow of oxygen and nutrients, leading to an energy-starved condition in the myocardium [3]. Therefore, reactive cardiac fibrosis is closely associated with physiological and pathological cardiac conditions. Reparative fibrosis, which occurs in response to the loss of viable myocardium and forms a scar, maintains the structural integrity of the ventricles. A balance between reactive and reparative fibrosis is important for the prevention of excessive and inappropriate cardiac dysfunction, particularly after CM death due to cardiac injury, such as MI [8].

Cardiac fibroblasts

Cardiac fibroblasts are flat, spindle-shaped cells located in the myocardium, with multiple processes originating from the cell body, and lack a basement membrane [3]. They play a major role in cardiac fibrosis by producing the ECM [3], and recent studies have demonstrated that mouse cardiac resident fibroblasts derived from the cells of the embryonic proepicardial organ (PEO) [9, 10] are major cell type producing the fibrotic ECM in a pressure overload model [11, 12]. However, other cell types have also been reported as origins of cardiac fibroblasts such as embryonic endothelium, which undergo endothelial-to-mesenchymal transition (EndMT) [13], circulating bone marrow cells [14], pericytes, and endothelial cells [15]. Because these cardiac fibroblasts lack a specific marker [11, 16, 17], investigating their regulation remains a challenging task.

Paracrine factors associated with cardiac fibrosis

Transforming growth factor-beta (TGF-β) and angiotensin II (Ang II) are major factors that regulate cardiac fibrosis (Fig. 1). The expression of the Ang II type 1 (AT1) receptor is greater in fibroblasts than in CMs [18]. The activation of the AT1 receptor in fibroblasts by Ang II leads to the secretion of TGF-β, which stimulates fibroblast proliferation and ECM protein synthesis in an autocrine manner [19, 20] and induces CM hypertrophy in a paracrine manner [18]. The infusion of a subpressor dose of Ang II into mice induces both cardiac hypertrophy and fibrosis [21]. Clinical studies have demonstrated that the blockade of RAS signaling by an ACE inhibitor or ARB effectively reduces cardiac fibrosis and remodeling and that this is independent of the blood pressure-lowering effect [22]. However, the concomitant use of aliskiren, the direct renin inhibitor, with an ACE inhibitor or ARB in post-MI patients with reduced left ventricular (LV) ejection fraction does not further attenuate LV remodeling but is instead associated with more adverse effects [23]. The effect of blocking RAS signaling for cardiac fibrosis may eventually reach a plateau, with an excessive RAS blockade increasing adverse effects. Therefore, the appropriate regulation of RAS signaling is important for the prevention of cardiac fibrosis without any adverse effects.

TGF-β plays an essential role in cardiac fibrosis. Treatment with a subpressor dose of Ang II does not induce cardiac hypertrophy or fibrosis in Tgfb1-deficient mice [24]. Therefore, Ang II-induced cardiac fibrosis is believed to be mediated, at least in part, by TGF-β. Although cardiac hypertrophy and fibrosis induced by TGF-β signaling are adaptive responses to acute stress [3], the inhibition of TGF-β signaling may be useful for treating cardiac fibrosis. Therapies targeting TGF-β signaling have already been investigated in various mammalian models. An intraperitoneal injection of a TGF-β neutralizing antibody into rats subjected to pressure overload not only inhibits fibroblast activation and cardiac fibrosis but also prevents diastolic dysfunction [25]. In contrast, in a mouse aortic banding-induced pressure overload model, an orally active, small molecule inhibitor of the TGF-β type I receptor (TGFBR1, also known as activin receptor-like kinase 5), SM16, attenuates the development of cardiac fibrosis but causes death because of rupture at the site of aortic banding [26]. Further studies using other models of hypertension-induced cardiac fibrosis, which are independent of aortic banding, should be conducted. An MI model has been used to evaluate the effects on cardiac fibrosis and function. The treatment of rats with GW788388, another orally active TGFBR1 inhibitor, 1 week after MI, significantly reduces TGF-β signaling and attenuates LV remodeling and systolic dysfunction [27]. However, an intraperitoneal injection of a TGF-β neutralizing antibody started either 1 week before or 5 days after MI increases mortality and exacerbates LV dilatation and contractile dysfunction in mice [28]. These results indicate that the consequences of inhibiting TGF-β are variable, depending on the disease model and the timing of inhibition, presumably because TGF-β signaling in the heart during stress plays different roles during the early and late phases of cardiovascular disease.

Cardiac hypertrophy induced by cardiac fibroblasts

Some paracrine factors from cardiac fibroblasts induce CM proliferation and/or hypertrophy. Embryonic, but not adult, cardiac fibroblasts secrete high levels of fibronectin, collagen III, and heparin-binding EGF-like growth factor in mice. These embryonic cardiac fibroblast-specific factors collaboratively interact and promote embryonic CM proliferation (Fig. 2) [29]. On the other hand, in adult mice, various paracrine factors secreted by cardiac fibroblasts, including TGF-β, induce CM hypertrophy but not proliferation; the Krüppel-like factor 5 (KLF5) transcription factor expressed in adult cardiac fibroblasts promotes CM hypertrophy and cardiac protection (Fig. 1). KLF5 transactivates the expression of platelet-derived growth factor A (PDGF-A), which leads to the migration and proliferation of fibroblasts in an autocrine manner. Further, KLF5 transactivates insulin-like growth factor-1 (IGF-1) to promote CM hypertrophy in a paracrine manner. The cardiac fibroblast-specific deletion of Klf5 ameliorates cardiac hypertrophy and fibrosis elicited by a moderate-intensity pressure overload [30]. On the other hand, a high-intensity pressure overload causes severe heart failure and early death in these mice. Furthermore, in wild-type mice, the administration of a peptide inhibitor of IGF-1 severely exacerbates heart failure induced by a high-intensity pressure overload. These results demonstrate that cardiac fibroblasts play pivotal roles in cardiac adaptive responses to pressure overload, which are, at least in part, regulated by IGF-1.

Fig. 2
figure2

Current strategies for cardiomyocyte regeneration. a Endogenous cardiac regeneration is primarily driven by the division of pre-existing CMs; currently, paracrine factors, the microenvironment, and small molecules that regulate this process are under investigation. b The direct reprogramming of cardiac fibroblasts into CMs is induced by a combination of cardiac-specific transcription factors and compounds. Investigations to improve the efficiency and maturity of generated CMs are currently in progress

Cellular sources of cardiac regeneration

The regenerative capacity greatly differs in adult mammalian organs, and organ-specific stem cells have been shown to contribute to regeneration in certain organs, such as the intestines, lungs, taste buds, and hair follicles [31,32,33,34]. In the mammalian heart, CMs rapidly proliferate during embryonic development; however, CMs exit the cell cycle, with the number of binucleated CMs increasing soon after birth [35]. One-day-old mice retain an adequate CM proliferative capacity and can completely regenerate CMs after cardiac injury, such as apical resection and MI. In response to cardiac injury, inflammation causes the proliferation of myofibroblasts and increases fibrosis in the regenerative area, but the myocardium is finally regenerated without fibrosis. Therefore, cardiac fibroblast-rich scar tissue may be an important component of cardiac repair in neonatal mice [17]. However, this efficient regenerative potential is lost within the first week of postnatal life [36, 37]; adult mice do not regenerate CMs adequately to compensate for the impaired cardiac function, inducing reparative fibrosis after injury instead. On the other hand, in certain lower vertebrates, such as teleost fish and urodele amphibians, adult CMs have sufficient regenerative capacity, and the myocardium can completely regenerate after injury without forming scar tissue [38, 39]. It is not known what causes these differences in the regenerative capacity of CMs between adult mammals and lower vertebrates. The fact that CMs in lower vertebrates are mononucleated and smaller in size with fewer myofibrils than those in adult mammals may be responsible for the differences observed in the CM regenerative capacity between these groups [40].

Recently, it has been shown that new CMs in adult humans are generated throughout life at a low rate (0.5–1% per year) [41]. Additional lines of evidence support the fact that the regeneration of adult mammalian CMs occurs at a low rate, decreasing with age but increasing with injury [42]. Various cell sources of endogenously regenerated CMs, such as pre-existing CMs, cardiac progenitor cells (CPCs), and cardiac fibroblasts, have been proposed, and lineage-tracing analyses (fate map) and/or cell transplantation studies have been used to determine the cellular source of regenerated CMs [5,44,45,46,47,48,49,, 4350]. Cardiac stem cells, such as c-kit-positive CPCs, islet 1-positive CPCs, stem cell antigen-1-positive CPCs, and cardiosphere-derived cells, have attracted considerable attention as cellular sources of regenerated CMs in the 2000s [44,45,46,47]; further, clinical trials using cardiac stem cells in patients with LV dysfunction have been conducted (Table 1). In the prospective, randomized CArdiosphere-Derived aUtologous stem CElls to reverse ventricUlar dySfunction (CADUCEUS) trial, an intracoronary infusion of cardiosphere-derived cells 1.5–3 months after MI reduced cardiac scar size; however, it did not improve LV systolic function after 1 year [51, 52]. In the Stem Cell Infusion in Patients with Ischemic cardiOmyopathy (SCIPIO) trial, post-MI patients with LV dysfunction who underwent coronary artery bypass grafting (CABG) were assigned to receive treatment with an intracoronary infusion of autologous c-kit-positive CPCs 4 ± 1 months after CABG. An intracoronary infusion of c-kit-positive CPCs effectively improved the LV systolic function and reduced the infarct size in these patients [53]. However, it is unclear whether c-kit-positive CPCs efficiently transdifferentiated into functional CMs [44, 54, 55]. To examine this possibility, Molkentin et al. performed lineage-tracing analysis after the labeling of c-kit-expressing cells in adult mice and demonstrated that the number of c-kit-positive cells that transdifferentiated into new CMs was low (<0.03%) even after cardiac injury, indicating that c-kit-positive CPCs are not a major source of newly generated CMs [56].

Table 1 Clinical trials using cardiac stem cells

Recent genetic fate-mapping experiments revealed that the regeneration of CMs occurs by the division of pre-existing CMs during normal aging at a low rate and that this process is enhanced in response to cardiac injury [5]. Therefore, it is accepted that new CMs are primarily derived from the division of pre-existing CMs. However, it remains unclear what prevents cell division in adult mammalian CMs whose endogenous regenerative capacity is insufficient to restore cardiac function after substantial damage. Therefore, growth factors, transcription factors, microRNAs, and small molecules that stimulate CM replication have been actively studied (Table 2) [37,58,59,60,61,62,63,64,65,66,, 5767]. Furthermore, the roles of the physiological and pathological environments of the heart in the regulation of cardiac regeneration have been studied with great detail (Fig. 1).

Table 2 Growth factors, transcription factors, microRNAs, and small molecules that stimulate CM replication

Impact of reactive oxygen species on CM regeneration

Recently, considerable attention has been given to the impact of reactive oxygen species (ROS) on cardiovascular diseases. Cardiac injury has been shown to increase the amount of ROS in the heart, which induces CM cell cycle arrest via the activation of responses to DNA damage (Fig. 2) [68, 69]. The inhibition of ROS by pretreatment with N-acetyl-L-cysteine has been shown to promote CM regeneration after ischemia–reperfusion injury even in 21-day-old mice [69]. In addition, the presence of oxygen in the environment has been reported to influence the production or scavenging of ROS and regeneration of CMs. Hyperoxic (100% oxygen) and hypoxic (15% oxygen) environments have been found to diminish and enhance CM proliferation, respectively, in neonatal mice with adequate CM regenerative capacities (Fig. 2) [69]. Furthermore, in adult mice, gradual exposure to severe hypoxia after MI, in which inspired oxygen is gradually decreased by 1% beginning 1 week after MI for 2 weeks, and then maintained at 7% for another 2 weeks, has been found to induce CM regeneration and coronary angiogenesis, resulting in improvements in the LV systolic function [70]. To evaluate the proliferation of hypoxic CMs in the adult heart, hypoxic CMs in αMHC-creERT2-ODD; R26R/tdTomato mice were genetically labeled at 2 months of age and fate mapped for 1 month under normal conditions; the results of this study demonstrated that labeled hypoxic CMs have a higher proliferative capacity than unlabeled CMs and can be a source of newly generated CMs [71].

Role of macrophages in cardiac regeneration

One-day-old mice can completely regenerate their hearts after MI injury. However, 14-day-old mice do not retain sufficient capacity for cardiac regeneration and cause fibrosis in response to cardiac injury. Clodronate liposome-mediated depletion of monocytes/macrophages in 1-day-old mice after MI reduces the angiogenic response, blocks the cardiac regenerative capacity, and induces cardiac fibrosis and dysfunction [72]. To identify the role of cardiac monocytes/macrophages in cardiac regeneration, immunophenotyping and gene expression profiling of cardiac monocytes/macrophages from 1-day-old and 14-day-old mice were isolated and compared after MI [72]. Regenerative macrophages from 1-day-old mice displayed both M1- and M2-associated gene transcription patterns and expressed more chemokines, proangiogenic factors, and oxidative stress responders, which may facilitate the formation of new myocardium than macrophages from 14-day-old mice.

Embryonic-derived resident cardiac macrophages (MHC-IIlowCCR2) and two types of resident cardiac macrophages (MHC-IIlowCCR2 and MHC-IIhighCCR2) are the major populations of monocytes/macrophages in neonatal and adult mouse hearts, respectively; monocytes (MHC-IIlowCCR2+) and monocyte-derived macrophages (MHC-IIhighCCR2+) are not abundant in either neonatal or adult hearts under normal physiological conditions [73]. To elucidate essential cardiac monocyte/macrophage subsets involved in cardiac regeneration, Lavine et al. used a diphtheria toxin receptor-mediated CM ablation mouse model [73], in which cardiac injury was induced without concomitant systemic inflammation. In response to diphtheria toxin receptor-mediated cardiac injury, the neonatal heart selectively expanded the population of embryonic-derived resident cardiac macrophages and cardiac dysfunction recovered to baseline. In contrast, in adult mice, the heart recruits CCR2+ pro-inflammatory monocytes and monocyte-derived macrophages and loses CCR2 resident cardiac macrophages after cardiac injury; cardiac function recovery was not observed. However, the administration of selective CCR2 inhibitors in adult mice after cardiac injury inhibited CCR2+ monocyte recruitment to the heart and preserved CCR2 resident cardiac macrophages, resulting in reduced inflammation and enhanced angiogenesis. Collectively, embryonic-derived resident cardiac macrophages are key mediators of angiogenesis, leading to cardiac regeneration in response to cardiac injury (Fig. 3).

Fig. 3
figure3

Angiogenic and fibrogenic responses during cardiac tissue injury and repair. Both MEndT and EndMT actively contribute to cardiac angiogenesis and fibrosis after cardiac injury. Embryonic macrophages can promote angiogenesis and subsequent cardiac regeneration in neonatal mice after cardiac injury, but infiltrate macrophages during adult cardiac injury do not

Interactions between endothelial cells and fibroblasts

EndMT is a fundamental cellular mechanism that regulates embryonic development and fibrotic diseases. During the embryonic development of the heart, the endocardium undergoes EndMT and forms an atrioventricular cushion: the primordial valves and septa of the adult heart [74]. Zeisberg et al. demonstrated that Tie1-expressing endothelial cells in the adult heart underwent EndMT and differentiated into fibroblasts during cardiac fibrosis in response to pressure overload [15]. Endothelial cells undergoing EndMT lost tight junctions that hold neighboring cells, gained the ability to move, and contributed to the total pool of cardiac fibroblasts. Although endothelial cells are not major origins of cardiac fibroblasts under normal conditions, inflammation induces EndMT of endothelial cells. As a result, approximately one-third of all cardiac fibroblasts originated from endothelial cells in the fibrotic heart in response to pressure overload. During this process, TGF-β1 induces EndMT, whereas bone morphogenic protein 7 (BMP-7) prevents EndMT and preserves the endothelial phenotype [15]. Therefore, the anti-fibrotic effects of recombinant human BMP-7 have been investigated. An intraperitoneal injection of recombinant human BMP-7 inhibited EndMT and the progression of cardiac fibrosis and improved diastolic cardiac function in a moderate-intensity pressure overload model. Furthermore, the inhibition of EndMT and cardiac fibrosis by recombinant human BMP-7 has been observed in a mouse model of chronic heart rejection caused by heterotopic heart transplantation with a class II major histocompatibility mismatch between donor and recipient [15].

Cardiac fibroblasts can undergo mesenchymal-to-endothelial transition (MEndT) immediately after ischemic cardiac injury [75]. Approximately 30% of fibroblasts in the injury zone undergo MEndT, and fibroblast-derived endothelial cells exhibit anatomical and functional characteristics of native endothelial cells and contribute to angiogenesis of the injured heart. p53, a transcription factor, regulates MEndT in cardiac fibroblasts [75]. The loss of p53 in Col1a2-expressing fibroblasts severely decreases the formation of fibroblast-derived endothelial cells, reduces the post-MI vascular area, and worsens cardiac function. Conversely, the stimulation of the p53 pathway after ischemic cardiac injury by an intraperitoneal injection of the small molecule: reactivation of p53 and induction of tumor cell apoptosis (RITA), which inhibits ubiquitin-mediated p53 degradation, augments MEndT, enhances angiogenesis, and improves cardiac function. However, although cardiac fibroblasts cultured in vitro under serum-free conditions have been found to form tubular structures resembling the endothelial cell architecture and express endothelial markers, cardiac fibroblasts cultured under serum-fed conditions fail to generate tubular structures, even when p53 is artificially overexpressed. This result suggests that p53 expression alone is insufficient to induce MEndT and that the microenvironment, growth factors, and other signals are involved in this process. Collectively, these close interactions between endothelial cells and fibroblasts regulate cardiac fibrosis and angiogenesis (Fig. 3), and the regulation of both EndMT and MEndT is a potential therapeutic target for enhancing cardiac repair.

Direct reprogramming of cardiac fibroblasts into CMs

In 2006, Takahashi and Yamanaka generated induced pluripotent stem (iPS) cells from mouse fibroblasts by introducing four factors: Oct3/4, Sox2, c-Myc, and Klf4 [76]. Subsequently, the direct reprogramming of fibroblasts by lineage-specific transcription factors into the primary functional cells of each organ, such as neurons, hepatocytes, and renal tubular epithelial cells, was accomplished [77,78,79,80]. Further, the direct reprogramming of mouse cardiac fibroblasts into CMs is induced by a combination of cardiac-specific transcription factors (Gata4, Mef2c, and Tbx5) in vitro [81]. Furthermore, endogenous cardiac fibroblasts were directly reprogrammed into CMs by the retrovirus-mediated delivery of cardiac-specific transcription factors in vivo, with such newly generated CMs reducing scar formation and cardiac dysfunction after MI [49, 50]. Several laboratories demonstrated that in vivo reprogramming yields higher quality of CMs than in vitro reprogramming. These results suggest that factors within the native microenvironment, such as the ECM, growth factors, local signals, and mechanical forces, enhance the maturity of CMs in the heart.

Although the direct reprogramming of cardiac fibroblasts into CMs in vivo can be a new cardiac regenerative therapy (Fig. 2), the efficiency of reprogramming is currently low to adequately improve cardiac function, and the mechanisms of reprogramming and properties of newly generated CMs have not yet been fully defined [82]. Therefore, the modification of transcription factors and induction of microRNAs have been studied, with the goal of improving the quality of cardiac reprogramming [50, 83]; the addition of factors that regulate the native microenvironment may enhance the efficacy of cardiac direct reprogramming.

Conclusions

Most types of cardiac cells, including cardiac fibroblasts, CMs, macrophages, and endothelial cells, regulate cardiac fibrosis in a coordinated manner; therefore, various elements and signals could be therapeutic targets for cardiac protection and the prevention of cardiac fibrosis. We commonly use ACE inhibitors or ARBs to block RAS signaling and inhibit cardiac fibrosis in patients with hypertension and cardiac diseases; however, there are few effective therapies that target other pathways involved in the prevention of cardiac fibrosis. Although targeting TGF-β signaling is a promising strategy, optimizing the appropriate timing and duration of treatment remains a challenging task.

Recently, it has been revealed that inflammatory and fibrotic responses to myocardial damage are essential for cardiac repair as well as cardiac regeneration; paracrine factors, the microenvironment, and small molecules that regulate these processes are all currently under investigation. Non-CMs, including macrophages, fibroblasts, and endothelial cells, cooperate with CMs to promote cardiac repair and regeneration. The regulation of the hypoxic environment and macrophage polarization may enhance crucial angiogenic responses involved in these processes. Further, the direct reprogramming of cardiac fibroblasts into functional CMs is an attractive strategy, and currently, investigations to improve the efficiency and maturity of generated CMs are in progress. Further research to unravel the regulatory mechanisms underlying cardiac fibrosis and regeneration will aid the development of therapeutic strategies to treat and prevent advanced heart failure.

Abbreviations

AAV:

Adeno-associated virus

ACE:

Angiotensin-converting enzyme

Ang II:

Angiotensin II

ARB:

Angiotensin receptor blocker

ARCM:

Adult rat cardiomyocytes

AT1:

Ang II type 1

BMP-7:

Bone morphogenic protein 7

BrdU:

5-bromo-2′-deoxyuridine

CABG:

Coronary artery bypass grafting

CM:

Cardiomyocyte

CPCs:

Cardiac progenitor cells

ECM:

Extracellular matrix

EdU:

5-ethynyl-2′- deoxyuridine

EF:

Ejection fraction

EndMT:

Endothelial-to-mesenchymal transition

FS:

Fractional shortening

I/R:

Ischemia/reperfusion

IGF-1:

Insulin-like growth factor-1

IP:

Intraperitoneal

KLF5:

Krüppel-like factor 5

LV:

Left ventricular

MEndT:

Mesenchymal-to-endothelial transition

MI:

Myocardial infarction

N/A:

Not available

NMCM:

Neonatal mouse cardiomyocytes

NRCM:

Neonatal rat cardiomyocytes

PDGF-A:

Platelet-derived growth factor-A

PEO:

Proepicardial organ

pH3:

Phospho-Histone H3

RAS:

Renin–angiotensin system

RITA:

Reactivation of p53 and induction of tumor cell apoptosis

ROS:

Reactive oxygen species

s.c.:

Subcutaneous

TGFBR1:

TGF-β type I receptor

TGF-β:

Transforming growth factor-beta

References

  1. 1.

    Akazawa H, Yabumoto C, Yano M, Kudo-Sakamoto Y, Komuro I. ARB and cardioprotection. Cardiovasc Drugs Ther. 2013;27:155–60.

  2. 2.

    Banerjee I, Fuseler JJW, Price RL, Borg TK, Baudino T, Jw F, et al. Determination of cell types and numbers during cardiac development in the neonatal and adult rat and mouse. Am Physiol. 2007;293:1883–91.

  3. 3.

    Takeda N, Manabe I. Cellular interplay between cardiomyocytes and nonmyocytes in cardiac remodeling. Int J Inflam. 2011;2011:535241.

  4. 4.

    Pellman J, Zhang J, Sheikh F. Myocyte-fibroblast communication in cardiac fibrosis and arrhythmias: mechanisms and model systems. J Mol Cell Cardiol. 2016;94:22–31.

  5. 5.

    Senyo SE, Steinhauser ML, Pizzimenti CL, Yang VK, Cai L, Wang M, et al. Mammalian heart renewal by pre-existing cardiomyocytes. Nature. 2013;493:433–6.

  6. 6.

    Anderson KR, Sutton MG, Lie JT. Histopathological types of cardiac fibrosis in myocardial disease. J Pathol. 1979;128:79–85.

  7. 7.

    Weber KT. Cardiac interstitium in health and disease: the fibrillar collagen network. J Am Coll Cardiol. 1989;13:1637–52.

  8. 8.

    Ramos G, Hofmann U, Frantz S. Myocardial fibrosis seen through the lenses of T-cell biology. J Mol Cell Cardiol. 2016;92:41–5.

  9. 9.

    Mikawa T, Gourdie RG. Pericardial mesoderm generates a population of coronary smooth muscle cells migrating into the heart along with ingrowth of the epicardial organ. Dev Biol. 1996;174:221–32.

  10. 10.

    Cai C-L, Martin JC, Sun Y, Cui L, Wang L, Ouyang K, et al. A myocardial lineage derives from Tbx18 epicardial cells. Nature. 2008;454:104–8.

  11. 11.

    Moore-Morris T, Guimarães-Camboa N, Banerjee I, Zambon AC, Kisseleva T, Velayoudon A, et al. Resident fibroblast lineages mediate pressure overload-induced cardiac fibrosis. J Clin Invest. 2014;124:2921–34.

  12. 12.

    Ali SR, Ranjbarvaziri S, Talkhabi M, Zhao P, Subat A, Hojjat A, et al. Developmental heterogeneity of cardiac fibroblasts does not predict pathological proliferation and activation. Circ Res. 2014;115:625–35.

  13. 13.

    Moore-morris T, Guimarães-camboa N, Banerjee I, Zambon AC, Kisseleva T, Velayoudon A, et al. Resident fibroblast lineages mediate pressure overload–induced cardiac fibrosis. J Clin Invest. 2014;124:1–14.

  14. 14.

    Van Amerongen MJ, Bou-Gharios G, Popa ER, Van Ark J, Petersen AH, Van Dam GM, et al. Bone marrow-derived myofibroblasts contribute functionally to scar formation after myocardial infarction. J Pathol. 2008;214:377–86.

  15. 15.

    Zeisberg EM, Tarnavski O, Zeisberg M, Dorfman AL, McMullen JR, Gustafsson E, et al. Endothelial-to-mesenchymal transition contributes to cardiac fibrosis. Nat Med. 2007;13:952–61.

  16. 16.

    Travers JG, Kamal FA, Robbins J, Yutzey KE, Blaxall BC. Cardiac fibrosis: the fibroblast awakens. Circ Res. 2016;118:1021–40.

  17. 17.

    Moore-Morris T, Cattaneo P, Puceat M, Evans SM. Origins of cardiac fibroblasts. J Mol Cell Cardiol. 2016;91:1–5.

  18. 18.

    Gray MO, Long CS, Kalinyak JE, Li HT, Karliner JS. Angiotensin II stimulates cardiac myocyte hypertrophy via paracrine release of TGF-β1 and endothelin-1 from fibroblasts. Cardiovasc Res. 1998;40:352–63.

  19. 19.

    Villarreal FJ, Kim NN, Ungab GD, Printz MP, Dillmann WH. Identification of functional angiotensin II receptors on rat cardiac fibroblasts. Circulation. 1993;88:2849–61.

  20. 20.

    Rosenkranz S. TGF-β1 and angiotensin networking in cardiac remodeling. Cardiovasc Res. 2004;63:423–32.

  21. 21.

    Harada K, Komuro I, Shiojima I, Hayashi D, Kudoh S, Mizuno T, et al. Pressure overload induces cardiac hypertrophy in angiotensin II type 1A receptor knockout mice. Circulation. 1998;97:1952–9.

  22. 22.

    Hoogwerf BJ. Renin-angiotensin system blockade and cardiovascular and renal protection. Am J Cardiol. 2010;105:30A–5A.

  23. 23.

    Solomon SD, Hee Shin S, Shah A, Skali H, Desai A, Kober L, et al. Effect of the direct renin inhibitor aliskiren on left ventricular remodelling following myocardial infarction with systolic dysfunction. Eur Heart J. 2011;32:1227–34.

  24. 24.

    Schultz JEJ, Witt SA, Glascock BJ, Nieman ML, Reiser PJ, Nix SL, et al. TGF-β1 mediates the hypertrophic cardiomyocyte growth induced by angiotensin II. J Clin Invest. 2002;109:787–96.

  25. 25.

    Kuwahara F, Kai H, Tokuda K, Kai M, Takeshita A, Egashira K, et al. Transforming growth factor-β function blocking prevents myocardial fibrosis and diastolic dysfunction in pressure-overloaded rats. Circulation. 2002;106:130–5.

  26. 26.

    Engebretsen KVT, Skårdal K, Bjørnstad S, Marstein HS, Skrbic B, Sjaastad I, et al. Attenuated development of cardiac fibrosis in left ventricular pressure overload by SM16, an orally active inhibitor of ALK5. J Mol Cell Cardiol. 2014;76:148–57.

  27. 27.

    Tan SM, Zhang Y, Connelly K, Gilbert RE, Kelly DJ. Targeted inhibition of activin receptor-like kinase 5 signaling attenuates cardiac dysfunction following myocardial infarction. Am J Physiol Heart Circ Physiol. 2010;298:H1415–25.

  28. 28.

    Frantz S, Hu K, Adamek A, Wolf J, Sallam A, Maier SKG, et al. Transforming growth factor beta inhibition increases mortality and left ventricular dilatation after myocardial infarction. Basic Res Cardiol. 2008;103:485–92.

  29. 29.

    Ieda M, Tsuchihashi T, Ivey KN, Ross RS, Hong TT, Shaw RM, et al. Cardiac fibroblasts regulate myocardial proliferation through β1 integrin signaling. Dev Cell. 2009;16:233–44.

  30. 30.

    Takeda N, Manabe I, Uchino Y, Eguchi K, Matsumoto S, Nishimura S, et al. Cardiac fibroblasts are essential for the adaptive response of the murine heart to pressure overload. J Clin Invest. 2010;120:254–65.

  31. 31.

    Takeda N, Jain R, LeBoeuf MR, Wang Q, Lu MM, Epstein J. Interconversion between intestinal stem cell populations in distinct niches. Science. 2011;334:1420–4.

  32. 32.

    Takeda N, Jain R, Leboeuf MR, Padmanabhan A, Wang Q, Li L, et al. Hopx expression defines a subset of multipotent hair follicle stem cells and a progenitor population primed to give rise to K6+ niche cells. Development. 2013;140:1655–64.

  33. 33.

    Takeda N, Jain R, Li D, Li L, Lu MM, Epstein J. Lgr5 identifies progenitor cells capable of taste bud regeneration after injury. PLoS ONE. 2013;8:1.

  34. 34.

    Jain R, Barkauskas CE, Takeda N, Bowie EJ, Aghajanian H, Wang Q, et al. Plasticity of Hopx(+) type I alveolar cells to regenerate type II cells in the lung. Nat Commun. 2015;6:6727.

  35. 35.

    Soonpaa MH, Kim KK, Pajak L, Franklin M, Field LJ. Cardiomyocyte DNA synthesis and binucleation during murine development. Am J Physiol. 1996;271:H2183–9.

  36. 36.

    Porrello ER, Mahmoud AI, Simpson E, Hill J, Richardson J, Olson EN, et al. Transient regenerative potential of the neonatal mouse heart. Science. 2011;331:1078–80.

  37. 37.

    Porrello ER, Mahmoud AI, Simpson E, Johnson B, Grinsfelder D, Canseco D, et al. Regulation of neonatal and adult mammalian heart regeneration by the miR-15 family. Proc Natl Acad Sci U S A. 2013;110:187–92.

  38. 38.

    Poss KD, Wilson LG, Keating MT. Heart regeneration in zebrafish. Science. 2002;298:2188–90.

  39. 39.

    Witman N, Murtuza B, Davis B, Arner A, Morrison JI. Recapitulation of developmental cardiogenesis governs the morphological and functional regeneration of adult newt hearts following injury. Dev Biol. 2011;354:67–76.

  40. 40.

    Kikuchi K, Poss KD. Cardiac regenerative capacity and mechanisms. Annu Rev Cell Dev Biol. 2012;28:719–41.

  41. 41.

    Bergmann O, Bhardwaj RD, Bernard S, Zdunek S, Barnabé-Heider F, Walsh S, et al. Evidence for cardiomyocyte renewal in humans. Science. 2009;324:98–102.

  42. 42.

    Garbern JC, Lee RT. Cardiac stem cell therapy and the promise of heart regeneration. Cell Stem Cell. 2013;12:689–98.

  43. 43.

    Van Berlo JH, Molkentin JD. An emerging consensus on cardiac regeneration. Nat Med. 2014;20:1386–93.

  44. 44.

    Beltrami AP, Barlucchi L, Torella D, Baker M, Limana F, Chimenti S, et al. Adult cardiac stem cells are multipotent and support myocardial regeneration. Cell. 2003;114:763–76.

  45. 45.

    Laugwitz K-L, Moretti A, Lam J, Gruber P, Chen Y, Woodard S, et al. Postnatal isl1+ cardioblasts enter fully differentiated cardiomyocyte lineages. Nature. 2005;433:647–53.

  46. 46.

    Oh H, Bradfute SB, Gallardo TD, Nakamura T, Gaussin V, Mishina Y, et al. Cardiac progenitor cells from adult myocardium: homing, differentiation, and fusion after infarction. Proc Natl Acad Sci U S A. 2003;100:12313–8.

  47. 47.

    Messina E, De Angelis L, Frati G, Morrone S, Chimenti S, Fiordaliso F, et al. Isolation and expansion of adult cardiac stem cells from human and murine heart. Circ Res. 2004;95:911–21.

  48. 48.

    Anversa P, Kajstura J, Rota M, Leri A. Regenerating new heart with stem cells. J Clin Invest. 2013;123:62–70.

  49. 49.

    Qian L, Huang Y, Spencer CI, Foley A, Vedantham V, Liu L, et al. In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes. Nature. 2012;485:593–8.

  50. 50.

    Song K, Nam Y-J, Luo X, Qi X, Tan W, Huang GN, et al. Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature. 2012;485:599–604.

  51. 51.

    Makkar RR, Smith RR, Cheng K, Malliaras K, Thomson LEJ, Berman D, et al. Intracoronary cardiosphere-derived cells for heart regeneration after myocardial infarction (CADUCEUS): a prospective, randomised phase 1 trial. Lancet. 2012;379:895–904.

  52. 52.

    Malliaras K, Makkar RR, Smith RR, Cheng K, Wu E, Bonow RO, et al. Intracoronary cardiosphere-derived cells after myocardial infarction: evidence of therapeutic regeneration in the final 1-year results of the CADUCEUS trial (CArdiosphere-derived aUtologous stem CElls to reverse ventricular dysfunction). J Am Coll Cardiol. 2014;63:110–22.

  53. 53.

    Bolli R, Chugh AR, D’Amario D, Loughran JH, Stoddard MF, Ikram S, et al. Cardiac stem cells in patients with ischaemic cardiomyopathy (SCIPIO): initial results of a randomised phase 1 trial. Lancet. 2011;378:1847–57.

  54. 54.

    Zaruba MM, Soonpaa M, Reuter S, Field LJ. Cardiomyogenic potential of C-kit + -expressing cells derived from neonatal and adult mouse hearts. Circulation. 2010;121:1992–2000.

  55. 55.

    Jesty SA, Steffey MA, Lee FK, Breitbach M, Hesse M, Reining S, et al. c-kit + precursors support postinfarction myogenesis in the neonatal, but not adult, heart. Proc Natl Acad Sci U S A. 2012;109:13380–5.

  56. 56.

    Van Berlo JH, Kanisicak O, Maillet M, Vagnozzi RJ, Karch J, Lin S-CJ, et al. C-kit + cells minimally contribute cardiomyocytes to the heart. Nature. 2014;509:337–41.

  57. 57.

    Bersell K, Arab S, Haring B, Kühn B. Neuregulin1/ErbB4 signaling induces cardiomyocyte proliferation and repair of heart injury. Cell. 2009;138:257–70.

  58. 58.

    Kühn B, Del Monte F, Hajjar RJ, Chang Y-S, Lebeche D, Arab S, et al. Periostin induces proliferation of differentiated cardiomyocytes and promotes cardiac repair. Nat Med. 2007;13:962–9.

  59. 59.

    Kubin T, Pöling J, Kostin S, Gajawada P, Hein S, Rees W, et al. Oncostatin M is a major mediator of cardiomyocyte dedifferentiation and remodeling. Cell Stem Cell. 2011;9:420–32.

  60. 60.

    Heallen T, Zhang M, Wang J, Bonilla-Claudio M, Klysik E, Johnson RL, et al. Hippo pathway inhibits Wnt signaling to restrain cardiomyocyte proliferation and heart size. Science. 2011;332:458–61.

  61. 61.

    Xin M, Kim Y, Sutherland LB, Murakami M, Qi X, McAnally J, et al. Hippo pathway effector Yap promotes cardiac regeneration. Proc Natl Acad Sci U S A. 2013;110:13839–44.

  62. 62.

    Lin Z, Von Gise A, Zhou P, Gu F, Ma Q, Jiang J, et al. Cardiac-specific YAP activation improves cardiac function and survival in an experimental murine MI model. Circ Res. 2014;115:354–63.

  63. 63.

    Porrello ER, Johnson B, Aurora AB, Simpson E, Nam YJ, Matkovich SJ, et al. MiR-15 family regulates postnatal mitotic arrest of cardiomyocytes. Circ Res. 2011;109:670–9.

  64. 64.

    Eulalio A, Mano M, Dal Ferro M, Zentilin L, Sinagra G, Zacchigna S, et al. Functional screening identifies miRNAs inducing cardiac regeneration. Nature. 2012;492:376–81.

  65. 65.

    Liu X, Xiao J, Zhu H, Wei X, Platt C, Damilano F, et al. miR-222 is necessary for exercise-induced cardiac growth and protects against pathological cardiac remodeling. Cell Metab. 2015;21:584–95.

  66. 66.

    Chen J, Huang ZP, Seok HY, Ding J, Kataoka M, Zhang Z, et al. Mir-17-92 cluster is required for and sufficient to induce cardiomyocyte proliferation in postnatal and adult hearts. Circ Res. 2013;112:1557–66.

  67. 67.

    Tseng AS, Engel FB, Keating MT. The GSK-3 inhibitor BIO promotes proliferation in mammalian cardiomyocytes. Chem Biol. 2006;13:957–63.

  68. 68.

    Tao G, Kahr PC, Morikawa Y, Zhang M, Rahmani M, Heallen TR, et al. Pitx2 promotes heart repair by activating the antioxidant response after cardiac injury. Nature. 2016;534:119–23.

  69. 69.

    Puente BN, Kimura W, Muralidhar S, Moon J, Amatruda JF, Phelps KL, et al. The oxygen-rich postnatal environment induces cardiomyocyte cell-cycle arrest through DNA damage response. Cell. 2014;157:565–79.

  70. 70.

    Nakada Y, Canseco DC, Thet S, Abdisalaam S, Asaithamby A, Santos CX, et al. Hypoxia induces heart regeneration in adult mice. Nature. 2017;541:222–7.

  71. 71.

    Kimura W, Xiao F, Canseco DC, Muralidhar S, Thet S, Zhang HM, et al. Hypoxia fate mapping identifies cycling cardiomyocytes in the adult heart. Nature. 2015;523:226–30.

  72. 72.

    Aurora AB, Porrello ER, Tan W, Mahmoud AI, Hill J, Bassel-Duby R, et al. Macrophages are required for neonatal heart regeneration. J Clin Invest. 2014;124:1382–92.

  73. 73.

    Lavine KJ, Epelman S, Uchida K, Weber KJ, Nichols CG, Schilling JD, et al. Distinct macrophage lineages contribute to disparate patterns of cardiac recovery and remodeling in the neonatal and adult heart. Proc Natl Acad Sci. 2014;111:16029–34.

  74. 74.

    Eisenberg LM, Markwald RR. Molecular regulation of atrioventricular valvuloseptal morphogenesis. Circ Res. 1995;77:1–6.

  75. 75.

    Ubil E, Duan J, Pillai ICL, Rosa-Garrido M, Wu Y, Bargiacchi F, et al. Mesenchymal-endothelial transition contributes to cardiac neovascularization. Nature. 2014;514:585–90.

  76. 76.

    Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006;126:663–76.

  77. 77.

    Kalani MYS, Martirosyan N. Direct conversion of fibroblasts to functional neurons. World Neurosurg. 2012;77:7–8.

  78. 78.

    Sekiya S, Suzuki A. Direct conversion of mouse fibroblasts to hepatocyte-like cells by defined factors. Nature. 2011;475:1–6.

  79. 79.

    Huang P, He Z, Ji S, Sun H, Xiang D, Liu C, et al. Induction of functional hepatocyte-like cells from mouse fibroblasts by defined factors. Nature. 2011;475:386–9.

  80. 80.

    Kaminski MM, Tosic J, Kresbach C, Engel H, Klockenbusch J, Müller A-L, et al. Direct reprogramming of fibroblasts into renal tubular epithelial cells by defined transcription factors. Nat Cell Biol. 2016;18:1269–80.

  81. 81.

    Ieda M, Fu JD, Delgado-Olguin P, Vedantham V, Hayashi Y, Bruneau BG, et al. Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell. 2010;142:375–86.

  82. 82.

    Sadahiro T, Yamanaka S, Ieda M. Direct cardiac reprogramming: progress and challenges in basic biology and clinical applications. Circ Res. 2015;116:1378–91.

  83. 83.

    Jayawardena TM, Finch EA, Zhang L, Zhang H, Hodgkinson CP, Pratt RE, et al. MicroRNA induced cardiac reprogramming in vivo evidence for mature cardiac myocytes and improved cardiac function. Circ Res. 2015;116:418–24.

Download references

Acknowledgements

We would like to express our sincere gratitude to all the researchers, collaborators, technical assistants, and secretaries for contributing to the studies cited in this review article. We also thank the grants supporting our research work.

Funding

There is no funding support for this review article.

Availability of data and materials

Not applicable.

Authors’ contributions

HH drafted the manuscript, and HH, NT, and IK revised it. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no competing interests.

Consent for publication

Not applicable.

Ethics approval

Not applicable.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Author information

Correspondence to Norifumi Takeda.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Hara, H., Takeda, N. & Komuro, I. Pathophysiology and therapeutic potential of cardiac fibrosis. Inflamm Regener 37, 13 (2017) doi:10.1186/s41232-017-0046-5

Download citation

Keywords

  • Cardiomyocytes
  • Cardiac fibroblasts
  • Cardiac regeneration
  • Direct reprogramming
  • Angiogenesis