Fig. 2

Par2 expression is maintained in WT hepatocytes and not in WT leukocytes 14 days after ConA injection. The core of large infiltrates is composed of apoptotic cells, which are CD45, Par2, and albumin negative. The apoptosis is indicated by the loss of the normal nuclei structure (note the ring-like circle of the Dapi staining, most apparent in C. The outer layer of the mononuclear infiltrate is composed of CD45+ leukocytes (shown in red). At day 14, these cells do not express Par2 and present normal nucleus. Healthy hepatocytes are not present in the infiltrate and observed by the uneven albumin staining of the infiltrate. At this stage of the ConA model, all apparent Par2+ cells express albumin (most observed in A and C’). The albumin+ staining is an indication of a functional hepatocyte. A Low, B middle, and C high magnifications. Scale bar = 50μm