Metabolic regulation by secreted phospholipase A2
© Sato et al. 2016
Received: 30 December 2015
Accepted: 10 May 2016
Published: 21 May 2016
Within the phospholipase A2 (PLA2) superfamily that hydrolyzes phospholipids to yield fatty acids and lysophospholipids, the secreted PLA2 (sPLA2) enzymes comprise the largest family that contains 11 isoforms in mammals. Individual sPLA2s exhibit unique distributions and specific enzymatic properties, suggesting their distinct biological roles. While sPLA2s have long been implicated in inflammation and atherosclerosis, it has become evident that they are involved in diverse biological events through lipid mediator-dependent or mediator-independent processes in a given microenvironment. In recent years, new biological aspects of sPLA2s have been revealed using their transgenic and knockout mouse models in combination with mass spectrometric lipidomics to unveil their target substrates and products in vivo. In this review, we summarize our current knowledge of the roles of sPLA2s in metabolic disorders including obesity, hepatic steatosis, diabetes, insulin resistance, and adipose tissue inflammation.
Historically, sPLA2s have long been implicated in inflammation and atherosclerosis. This idea stems from the observations that sPLA2-IIA, a prototypic “inflammatory sPLA2,” is induced during inflammation  and that hydrolysis of low-density lipoprotein (LDL) by sPLA2s gives rise to pro-atherogenic LDL, which promotes macrophage foam cell formation in vitro [16, 17]. Indeed, subsequent genetic and pharmacological approaches support the pro-inflammatory or atherosclerotic roles of sPLA2s [10–14]. However, the regulatory roles of sPLA2s in metabolic disorders including obesity and insulin resistance have not yet been fully elucidated. Recently, it has become clear that several sPLA2s are expressed in the adipose tissue or gastrointestinal (GI) tract and have variable influences on systemic metabolic states [18–20]. Here, we will make an overview of the novel biological roles of sPLA2s and the lipid pathways underlying metabolic regulation, as revealed by sophisticated knockout and lipidomics techniques.
sPLA2-V, a “metabolic sPLA2”
Metabolic syndrome is increasing at an explosive rate worldwide due to a pandemic of obesity associated with diabetes, insulin resistance, non-alcoholic fatty liver disease, and hyperlipidemia . The mechanisms connecting obesity to insulin resistance include an elevated level of circulating lipids, ectopic lipid deposition leading to lipotoxicity, and chronic inflammation in metabolically active tissues . Obesity arises through the dysregulations of intracellular lipid metabolism or extracellular lipid partitioning among tissues, and the perturbation of intracellular/extracellular lipases variably and often profoundly affect obesity and insulin resistance [23–26]. For instance, lipoprotein lipase is an obesity susceptibility factor showing an inverse relationship between its activity and obesity-related traits in humans . The imbalanced accumulation of LDL in favor of high-density lipoprotein (HDL) is a critical risk factor not only for atherosclerosis but also for insulin intolerance . As lipoprotein particles are shielded by phospholipids, aberrant lipoprotein phospholipid metabolism could also influence lipid partitioning and thereby obesity.
Among the sPLA2 isoforms, sPLA2-V potently hydrolyzes phospholipids in lipoproteins (LDL > HDL) in vitro . However, studies using Pla2g5 −/− mice have failed to demonstrate the participation of sPLA2-V in LDL metabolism in atherosclerosis models [11, 28]. Except for studies using sPLA2-overexpressing transgenic mice [17, 29, 30], no reports have firmly established whether endogenous sPLA2s affect lipoprotein metabolism in vivo. In a microarray search for unique lipase-related genes whose expressions are associated with obesity, we recently found that sPLA2-V (and sPLA2-IIE; see below) is robustly induced in adipocytes of obese mice . This sPLA2-V induction is dependent on adipogenesis plus endoplasmic reticulum (ER) stress. Because of this property plus the fact that sPLA2-V is constitutively expressed at relatively high levels in several metabolic tissues such as the heart and skeletal muscle, we refer to sPLA2-V as a “metabolic sPLA2.”
Notably, when fed a high-fat diet (HFD), Pla2g5 −/− mice display hyperlipidemia with higher plasma levels of LDL, increased obesity and hepatic steatosis, and lower insulin sensitivity . Furthermore, the adipose tissues in Pla2g5 −/− mice show a greater infiltration of M1 macrophages and a higher expression of pro-inflammatory cytokines. Thus, sPLA2-V plays anti-obesity and anti-inflammatory roles in the context of metabolic disorders. Lipidomics have revealed that sPLA2-V secreted from hypertrophic adipocytes preferentially hydrolyzes phosphatidylcholine (PC) in fat-overladen LDL to release unsaturated fatty acids (e.g., oleate and linoleate) in vivo . As such, the increased LDL lipid levels in Pla2g5 −/− mice could impact on adipocyte hypertrophy and the fatty liver. Furthermore, in accordance with the alterations in LDL phospholipids, the levels of free oleate and linoleate are lower in the adipose tissue of HFD-fed Pla2g5 −/− mice than in that of WT mice. These unsaturated fatty acids released by sPLA2-V dampen the M1 macrophage polarization by saturated fatty acids (e.g., palmitate) likely through the attenuation of ER stress. This mechanism fits with the view that sPLA2-V has an apparent, even if not strict, substrate preference for PC bearing a fatty acid with a low degree of unsaturation.
Notably, in humans, Pla2g5 gene polymorphisms correlate with the LDL levels in subjects with type 2 diabetes or obesity [35, 36]. The in vitro sPLA2-V susceptibility of LDL from patients with type 2 diabetes is greater than that of LDL from healthy controls . Moreover, the Pla2g5 expression in the human visceral adipose tissue inversely correlates with LDL plasma levels . These results imply a human relevance for the metabolic role of sPLA2-V.
sPLA2-IIE, another “metabolic sPLA2”
We also found that sPLA2-IIE, which remained a functionally orphan sPLA2 for more than a decade, acts as another “metabolic” sPLA2 that is induced in hypertrophic adipocytes . An adipogenic stimulus is sufficient for induction of sPLA2-IIE in adipocytes. Pla2g2e −/− mice are modestly protected from diet-induced obesity, hepatic steatosis, and hyperlipidemia. In contrast to sPLA2-V, which hydrolyzes PC in LDL to selectively release oleate and linoleate (see above), sPLA2-IIE preferentially hydrolyzes minor lipoprotein phospholipids, phosphatidylserine (PS), and phosphatidylethanolamine (PE), with no apparent fatty acid selectivity. As such, sPLA2-IIE alters the lipid composition of lipoproteins, thereby moderately affecting the lipid accumulation in the adipose tissue and liver.
Although the molecular mechanism that links lipoprotein PS/PE hydrolysis with obesity still remains unclear, this study revealed for the first time the importance of these minor lipoprotein phospholipids in metabolic regulation. As the increase of the negative charges in lipoproteins by oxidative modification renders the particles smaller, the increase of the anionic phospholipids (e.g., PS) in lipoproteins by the absence of sPLA2-IIE may also afford a similar effect. Alternatively, lysophosphatidylethanolamine or lysophosphatidylserine produced by sPLA2-IIE might have some metabolic effects, a possibility that awaits future studies. Collectively, these results underscore the physiological relevance of lipoprotein hydrolysis by distinct sPLA2s and highlight the importance of “metabolic sPLA2s” as the integrated regulators of metabolic responses (Fig. 2).
On the other hand, another study has recently reported that Pla2g2e −/− mice accumulate more epididymal fat as they age . During adipogenesis, the genetic deletion or siRNA knockdown of sPLA2-IIE increases the triglyceride in adipocytes, while its overexpression or exogenous addition facilitates lipolysis. Although the reason for the discrepancy between the two studies is unclear, it might have arisen from different experimental conditions (HFD versus chow diets or female versus male mice) in different animal facilities.
sPLA2-IB, a “digestive sPLA2”
Systemic lipid metabolism is often affected by the digestion and absorption of dietary lipids in the GI tract. sPLA2-IB is synthesized by pancreatic acinar cells, and after secretion as a zymogen into pancreatic juice, an N-terminal propeptide of the inactive zymogen is cleaved by trypsin to yield an active enzyme in the duodenum. The hydrolysis of PC by sPLA2-IB is greatly accelerated in the presence of a low concentration of detergent such as deoxycholate . This property appears to be physiologically important since the digestion of dietary phospholipids by sPLA2-IB occurs in the presence of bile acid in the GI tract.
Pla2g1b −/− mice show resistance to obesity, lower plasma insulin and leptin levels, and improved glucose tolerance when fed a high-fat/carbohydrate diet . These phenotypes of Pla2g1b −/− mice are most likely due to a marked reduction in the hydrolysis of dietary and biliary PC and thereby in the production and absorption of lysophosphatidylcholine (LPC) in the GI tract. The increased intestinal absorption of LPC promotes postprandial hyperglycemia by inhibiting the glucose uptake by the liver and muscle, and accordingly, the absence of sPLA2-IB reduces the postprandial LPC levels, leading to improved insulin sensitivity and hepatic fatty acid oxidation [41, 42]. It is noteworthy that Pla2g1b −/− mice on a Ldlr −/− background are protected from body weight gain and atherosclerosis in response to a hypercaloric diet  and that the oral administration of the sPLA2 inhibitor methyl indoxam along with a diabetogenic diet effectively suppresses diet-induced obesity and diabetes in mice likely through the prevention of the intestinal digestion of dietary and biliary PC by sPLA2-IB . In further support of these observations, pancreatic acinar cell-specific Pla2g1b-transgenic mice develop more severe obesity and insulin resistance . These results suggest that the inhibition of sPLA2-IB, a “digestive sPLA2,” may be an effective oral therapeutic option for the treatment of diet-induced obesity and diabetes.
Complex and enigmatic roles of sPLA2-X in metabolism
Lastly, we briefly summarize the possible metabolic roles of sPLA2-X, although details remain uncertain because of the fact that conflicting results have been obtained. Like sPLA2-IB, sPLA2-X also has an N-terminal propeptide, and its proteolytic removal leads to the full activation of the enzyme. A series of studies have provided some insights into the functional link of sPLA2-X-released polyunsaturated fatty acids to lipid-sensing nuclear receptor signaling. Macrophages from Pla2g10 −/− mice show an increased expression of the cholesterol efflux transporters ABCA1 and ABCG1, and this effect appears to be dependent on the suppression of the liver X receptor (LXR) by sPLA2-X-released polyunsaturated fatty acids . Moreover, the increased cholesterol content of the lipid rafts in Pla2g10 −/− macrophages leads to significant reduction of endotoxin-induced inflammation . The sPLA2-X-dependent suppression of LXR can also occur in the adipose tissue, where Pla2g10 deficiency facilitates adipogenesis and obesity , and in the adrenal glands, where its deficiency promotes corticosteroidogenesis through the activation of steroidogenic acute regulatory protein . In the latter case, pro-sPLA2-X is proteolytically processed to a mature, active form by the protein convertases furin and PCSK6, which are induced by the adrenocorticotropic hormone, in adrenal cells . However, as far as we have been able to examine, the Pla2g10 expression in mouse macrophages and the adipose tissue is very low, arguing against the above observations. Rather, we prefer the idea that sPLA2-X might be expressed in a limited subset of these cells or supplied from proximal or distal cells in a paracrine manner.
On the other hand, we have shown that sPLA2-X is expressed abundantly in GI-lining cells and participates in phospholipid digestion . Accordingly, Pla2g10 −/− mice display a reduced age-associated adiposity and improved insulin sensitivity in the skeletal muscle, likely through a mechanism reminiscent of that in Pla2g1b −/− mice. Thus, the two “digestive sPLA2s” (IB and X) may spatiotemporally control the hydrolysis of dietary and biliary phospholipids and thereby the absorption of their hydrolytic products, depending on the quantity and quality of the dietary and biliary fat input. As in the case of Pla2g2e −/− mice (see above), the opposite phenotypes of Pla2g10 −/− mice observed in different studies might have been due to differences in the experimental models or housing conditions employed, and further studies will be necessary to clarify more definitively the roles and mechanistic actions of sPLA2-X in metabolism.
It has been recently reported that the glucose-stimulated insulin secretion by islet β cells is augmented in Pla2g10 −/− mice, underscoring a novel metabolic role of sPLA2-X . Mechanistically, sPLA2-X negatively regulates insulin secretion by augmenting the cyclooxygenase-2-dependent prostaglandin E2 production. In this scenario, targeting sPLA2-X may be an effective therapeutic option for enhancing β cell function in the treatment of diabetes.
It is now obvious that at least four sPLA2s are involved in metabolic regulation through distinct mechanisms, as summarized below. sPLA2-V is induced in hypertrophic adipocytes by obesity-associated ER stress and hydrolyzes PC in hyperlipidemic LDL to facilitate the skewing of macrophages from M1 to M2 subsets, thereby conferring protection from adipose tissue inflammation, insulin resistance, obesity, hepatic steatosis, and hyperlipidemia. The saturated fatty acids supplied abundantly from adipocytes trigger the M1 polarization of macrophages, which is counterregulated by the sPLA2-V-driven unsaturated fatty acids from LDL. sPLA2-IIE is induced in adipocytes in accordance with adipogenesis and hydrolyzes PE and PS in lipoproteins, eventually promoting fat storage in the adipose tissue and liver. sPLA2-IB, a pancreatic sPLA2 that is secreted into the GI lumen, hydrolyzes dietary and biliary phospholipids to promote lipid digestion and absorption, which is associated with obesity and hepatic insulin resistance. sPLA2-X variably affects metabolism possibly through the production of polyunsaturated fatty acids that modify the LXR signaling in the adipose tissue, through the digestion of the dietary and biliary phospholipids in the gut, or through the generation of prostaglandin E2 that suppresses insulin secretion in the pancreatic islet. In addition, sPLA2-IIA is abundantly expressed in the human and rat adipose tissues in obesity and the pharmacological inhibition of this isoform attenuates the adipose tissue inflammation in rats [18, 52]. It remains possible that other sPLA2 isoforms may also participate in metabolic regulation, and this issue is now under investigation. Together, these studies have brought about a paradigm shift toward a better understanding of the biological roles of this extracellular lipolytic enzyme family as coordinators of metabolism.
We thank Dr. A. Kumoanogoh (University of Osaka, Japan) for providing an opportunity to write this manuscript.
This work was supported by a grant-in-aid for scientific research from the Ministry of Education, Science, Culture, Sports and Technology of Japan and AMED-CREST from the Japan Agency for Medical Research and Development.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Lambeau G, Gelb MH. Biochemistry and physiology of mammalian secreted phospholipases A2. Annu Rev Biochem. 2008;77:495–520.View ArticlePubMedGoogle Scholar
- Murakami M, Taketomi Y, Girard C, Yamamoto K, Lambeau G. Emerging roles of secreted phospholipase A2 enzymes: lessons from transgenic and knockout mice. Biochimie. 2010;92:561–82.View ArticlePubMedGoogle Scholar
- Dennis EA, Cao J, Hsu YH, Magrioti V, Kokotos G. Phospholipase A2 enzymes: physical structure, biological function, disease implication, chemical inhibition, and therapeutic intervention. Chem Rev. 2011;111:6130–85.View ArticlePubMedPubMed CentralGoogle Scholar
- Murakami M, Taketomi Y, Miki Y, Sato H, Hirabayashi T, Yamamoto K. Recent progress in phospholipase A2 research: from cells to animals to humans. Prog Lipid Res. 2011;50:152–92.View ArticlePubMedGoogle Scholar
- Murakami M, Sato H, Miki Y, Yamamoto K, Taketomi Y. A new era of secreted phospholipase A2. J Lipid Res. 2015;56:1248–61.PubMedGoogle Scholar
- Uozumi N, Kume K, Nagase T, Nakatani N, Ishii S, Tashiro F, et al. Role of cytosolic phospholipase A2 in allergic response and parturition. Nature. 1997;390:618–22.View ArticlePubMedGoogle Scholar
- Taketomi Y, Ueno N, Kojima T, Sato H, Murase R, Yamamoto K, et al. Mast cell maturation is driven via a group III phospholipase A2-prostaglandin D2-DP1 receptor paracrine axis. Nat Immunol. 2013;14:554–63.View ArticlePubMedPubMed CentralGoogle Scholar
- Sato H, Taketomi Y, Isogai Y, Miki Y, Yamamoto K, Masuda S, et al. Group III secreted phospholipase A2 regulates epididymal sperm maturation and fertility in mice. J Clin Invest. 2010;120:1400–14.View ArticlePubMedPubMed CentralGoogle Scholar
- Yamamoto K, Taketomi Y, Isogai Y, Miki Y, Sato H, Masuda S, et al. Hair follicular expression and function of group X secreted phospholipase A2 in mouse skin. J Biol Chem. 2011;286:11616–31.View ArticlePubMedPubMed CentralGoogle Scholar
- Boilard E, Lai Y, Larabee K, Balestrieri B, Ghomashchi F, Fujioka D, et al. A novel anti-inflammatory role for secretory phospholipase A2 in immune complex-mediated arthritis. EMBO Mol Med. 2010;2:172–87.View ArticlePubMedPubMed CentralGoogle Scholar
- Bostrom MA, Boyanovsky BB, Jordan CT, Wadsworth MP, Taatjes DJ, de Beer FC, et al. Group V secretory phospholipase A2 promotes atherosclerosis: evidence from genetically altered mice. Arterioscler Thromb Vasc Biol. 2007;27:600–6.View ArticlePubMedGoogle Scholar
- Henderson Jr WR, Chi EY, Bollinger JG, Tien YT, Ye X, Castelli L, et al. Importance of group X-secreted phospholipase A2 in allergen-induced airway inflammation and remodeling in a mouse asthma model. J Exp Med. 2007;204:865–77.View ArticlePubMedPubMed CentralGoogle Scholar
- Muñoz NM, Meliton AY, Arm JP, Bonventre JV, Cho W, Leff AR. Deletion of secretory group V phospholipase A2 attenuates cell migration and airway hyperresponsiveness in immunosensitized mice. J Immunol. 2007;179:4800–7.View ArticlePubMedGoogle Scholar
- Yamamoto K, Miki Y, Sato M, Taketomi Y, Nishito Y, Taya C, et al. The role of group IIF-secreted phospholipase A2 in epidermal homeostasis and hyperplasia. J Exp Med. 2015;212:1901–19.View ArticlePubMedPubMed CentralGoogle Scholar
- Seilhamer JJ, Pruzanski W, Vadas P, Plant S, Miller JA, Kloss J, et al. Cloning and recombinant expression of phospholipase A2 present in rheumatoid arthritic synovial fluid. J Biol Chem. 1989;264:5335–8.PubMedGoogle Scholar
- Hanasaki K, Yamada K, Yamamoto S, Ishimoto Y, Saiga A, Ono T, et al. Potent modification of low density lipoprotein by group X secretory phospholipase A2 is linked to macrophage foam cell formation. J Biol Chem. 2002;277:29116–24.View ArticlePubMedGoogle Scholar
- Sato H, Kato R, Isogai Y, Saka G, Ohtsuki M, Taketomi Y, et al. Analyses of group III secreted phospholipase A2 transgenic mice reveal potential participation of this enzyme in plasma lipoprotein modification, macrophage foam cell formation, and atherosclerosis. J Biol Chem. 2008;283:33483–97.View ArticlePubMedPubMed CentralGoogle Scholar
- Sato H, Taketomi Y, Ushida A, Isogai Y, Kojima T, Hirabayashi T, et al. The adipocyte-inducible secreted phospholipases PLA2G5 and PLA2G2E play distinct roles in obesity. Cell Metab. 2014;20:119–32.View ArticlePubMedPubMed CentralGoogle Scholar
- Sato H, Isogai Y, Masuda S, Taketomi Y, Miki Y, Kamei D, et al. Physiological roles of group X-secreted phospholipase A2 in reproduction, gastrointestinal phospholipid digestion, and neuronal function. J Biol Chem. 2011;286:11632–48.View ArticlePubMedPubMed CentralGoogle Scholar
- Hui DY. Phospholipase A2 enzymes in metabolic and cardiovascular diseases. Curr Opin Lipidol. 2012;23:235–40.View ArticlePubMedPubMed CentralGoogle Scholar
- Despres JP, Lemieux I. Abdominal obesity and metabolic syndrome. Nature. 2006;444:881–7.View ArticlePubMedGoogle Scholar
- Hotamisligil GS. Inflammation and metabolic disorders. Nature. 2006;444:860–7.View ArticlePubMedGoogle Scholar
- Chen Y, Zhu J, Lum PY, Yang X, Pinto S, MacNeil DJ, et al. Variations in DNA elucidate molecular networks that cause disease. Nature. 2008;452:429–35.View ArticlePubMedPubMed CentralGoogle Scholar
- Chiu HK, Qian K, Ogimoto K, Morton GJ, Wisse BE, Agrawal N, et al. Mice lacking hepatic lipase are lean and protected against diet-induced obesity and hepatic steatosis. Endocrinology. 2010;151:993–1001.View ArticlePubMedPubMed CentralGoogle Scholar
- Haemmerle G, Lass A, Zimmermann R, Gorkiewicz G, Meyer C, Rozman J, et al. Defective lipolysis and altered energy metabolism in mice lacking adipose triglyceride lipase. Science. 2006;312:734–7.View ArticlePubMedGoogle Scholar
- Wang H, Knaub LA, Jensen DR, Young Jung D, Hong EG, Ko HJ, et al. Skeletal muscle-specific deletion of lipoprotein lipase enhances insulin signaling in skeletal muscle but causes insulin resistance in liver and other tissues. Diabetes. 2009;58:116–24.View ArticlePubMedPubMed CentralGoogle Scholar
- Avramoglu RK, Basciano H, Adeli K. Lipid and lipoprotein dysregulation in insulin resistant states. Clin Chim Acta. 2006;368:1–19.View ArticlePubMedGoogle Scholar
- Boyanovsky B, Zack M, Forrest K, Webb NR. The capacity of group V sPLA2 to increase atherogenicity of ApoE−/− and LDLR−/− mouse LDL in vitro predicts its atherogenic role in vivo. Arterioscler Thromb Vasc Biol. 2009;29:532–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Ivandic B, Castellani LW, Wang XP, Qiao JH, Mehrabian M, Navab M, et al. Role of group II secretory phospholipase A2 in atherosclerosis: 1. Increased atherogenesis and altered lipoproteins in transgenic mice expressing group IIa phospholipase A2. Arterioscler Thromb Vasc Biol. 1999;19:1284–90.View ArticlePubMedGoogle Scholar
- Yamamoto K, Isogai Y, Sato H, Taketomi Y, Murakami M. Secreted phospholipase A2, lipoprotein hydrolysis, and atherosclerosis: integration with lipidomics. Anal Bioanal Chem. 2011;400:1829–42.View ArticlePubMedPubMed CentralGoogle Scholar
- Ohta S, Imamura M, Xing W, Boyce JA, Balestrieri B. Group V secretory phospholipase A2 is involved in macrophage activation and is sufficient for macrophage effector functions in allergic pulmonary inflammation. J Immunol. 2013;190:5927–38.View ArticlePubMedPubMed CentralGoogle Scholar
- Rubio JM, Rodríguez JP, Gil-de-Gómez L, Guijas C, Balboa MA, Balsinde J. Group V secreted phospholipase A2 is upregulated by IL-4 in human macrophages and mediates phagocytosis via hydrolysis of ethanolamine phospholipids. J Immunol. 2015;194:3327–39.View ArticlePubMedGoogle Scholar
- Odegaard JI, Chawla A. The immune system as a sensor of the metabolic state. Immunity. 2013;38:644–54.View ArticlePubMedPubMed CentralGoogle Scholar
- Balestrieri B, Maekawa A, Xing W, Gelb MH, Katz HR, Arm JP. Group V secretory phospholipase A2 modulates phagosome maturation and regulates the innate immune response against Candida albicans. J Immunol. 2009;182:4891–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Sergouniotis PI, Davidson AE, Mackay DS, Lenassi E, Li Z, Robson AG, et al. Biallelic mutations in PLA2G5, encoding group V phospholipase A2, cause benign fleck retina. Am J Hum Genet. 2011;89:782–91.View ArticlePubMedPubMed CentralGoogle Scholar
- Wootton PT, Arora NL, Drenos F, Thompson SR, Cooper JA, Stephens JW, et al. Tagging SNP haplotype analysis of the secretory PLA2-V gene, PLA2G5, shows strong association with LDL and oxLDL levels, suggesting functional distinction from sPLA2-IIA: results from the UDACS study. Hum Mol Genet. 2007;16:1437–44.View ArticlePubMedGoogle Scholar
- Pettersson C, Fogelstrand L, Rosengren B, Stahlman S, Hurt-Camejo E, Fagerberg B, et al. Increased lipolysis by secretory phospholipase A2 group V of lipoproteins in diabetic dyslipidaemia. J Intern Med. 2008;264:155–65.View ArticlePubMedGoogle Scholar
- Zhi H, Qu L, Wu F, Chen L, Tao J. Group IIE secretory phospholipase A2 regulates lipolysis in adipocytes. Obesity (Silver Spring). 2015;23:760–8.View ArticleGoogle Scholar
- Jain MK, Egmond MR, Verheij HM, Apitz-Castro R, Dijkman R, De Haas GH. Interaction of phospholipase A2 and phospholipid bilayers. Biochim Biophys Acta. 1982;688:341–8.View ArticlePubMedGoogle Scholar
- Huggins KW, Boileau AC, Hui DY. Protection against diet-induced obesity and obesity-related insulin resistance in Group 1B PLA2-deficient mice. Am J Physiol Endocrinol Metab. 2002;283:E994–E1001.View ArticlePubMedGoogle Scholar
- Labonté ED, Kirby RJ, Schildmeyer NM, Cannon AM, Huggins KW, Hui DY. Group 1B phospholipase A2-mediated lysophospholipid absorption directly contributes to postprandial hyperglycemia. Diabetes. 2006;55:935–41.View ArticlePubMedPubMed CentralGoogle Scholar
- Labonté ED, Pfluger PT, Cash JG, Kuhel DG, Roja JC, Magness DP, et al. Postprandial lysophospholipid suppresses hepatic fatty acid oxidation: the molecular link between group 1B phospholipase A2 and diet-induced obesity. FASEB J. 2010;24:2516–24.View ArticlePubMedPubMed CentralGoogle Scholar
- Hollie NI, Konaniah ES, Goodin C, Hui DY. Group 1B phospholipase A2 inactivation suppresses atherosclerosis and metabolic diseases in LDL receptor-deficient mice. Atherosclerosis. 2014;234:377–80.View ArticlePubMedPubMed CentralGoogle Scholar
- Hui DY, Cope MJ, Labonté ED, Chang HT, Shao J, Goka E, et al. The phospholipase A2 inhibitor methyl indoxam suppresses diet-induced obesity and glucose intolerance in mice. Br J Pharmacol. 2009;157:1263–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Cash JG, Kuhel DG, Goodin C, Hui DY. Pancreatic acinar cell-specific overexpression of group 1B phospholipase A2 exacerbates diet-induced obesity and insulin resistance in mice. Int J Obes (Lond). 2011;35:877–81.View ArticleGoogle Scholar
- Shridas P, Bailey WM, Gizard F, Oslund RC, Gelb MH, Bruemmer D, et al. Group X secretory phospholipase A2 negatively regulates ABCA1 and ABCG1 expression and cholesterol efflux in macrophages. Arterioscler Thromb Vasc Biol. 2010;30:2014–21.View ArticlePubMedPubMed CentralGoogle Scholar
- Shridas P, Bailey WM, Talbott KR, Oslund RC, Gelb MH, Webb NR. Group X secretory phospholipase A2 enhances TLR4 signaling in macrophages. J Immunol. 2011;187:482–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Li X, Shridas P, Forrest K, Bailey W, Webb NR. Group X secretory phospholipase A2 negatively regulates adipogenesis in murine models. FASEB J. 2010;24:4313–24.View ArticlePubMedPubMed CentralGoogle Scholar
- Shridas P, Bailey WM, Boyanovsky BB, Oslund RC, Gelb MH, Webb NR. Group X secretory phospholipase A2 regulates the expression of steroidogenic acute regulatory protein (StAR) in mouse adrenal glands. J Biol Chem. 2010;285:20031–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Layne JD, Shridas P, Webb NR. Ectopically expressed pro-group X secretory phospholipase A2 is proteolytically activated in mouse adrenal cells by furin-like proprotein convertases: implications for the regulation of adrenal steroidogenesis. J Biol Chem. 2015;290:7851–60.View ArticlePubMedPubMed CentralGoogle Scholar
- Shridas P, Zahoor L, Forrest KJ, Layne JD, Webb NR. Group X secretory phospholipase A2 regulates insulin secretion through a cyclooxygenase-2-dependent mechanism. J Biol Chem. 2014;289(40):27410–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Iyer A, Lim J, Poudyal H, Reid RC, Suen JY, Webster J, et al. An inhibitor of phospholipase A2 group IIA modulates adipocyte signaling and protects against diet-induced metabolic syndrome in rats. Diabetes. 2012;61:2320–9.View ArticlePubMedPubMed CentralGoogle Scholar